# install cell skipped during CI (deps preinstalled into system Python)

from dandi.dandiapi import DandiAPIClient

client = DandiAPIClient.for_dandi_instance("dandi")

dandiset_id = "001084"
file_path = "sub-DL18/sub-DL18_ses-211110_image+ophys.nwb"

with DandiAPIClient() as client:
    asset = client.get_dandiset(dandiset_id, 'draft').get_asset_by_path(file_path)
    s3_url = asset.get_content_url(follow_redirects=1, strip_query=True)

import h5py
from pynwb import NWBHDF5IO
import remfile

# We stream the file using remfile and open it with h5py and pynwb
file = remfile.File(s3_url)
h5_file = h5py.File(file, "r")
io = NWBHDF5IO(file=h5_file, load_namespaces=True)

nwbfile = io.read()

/opt/hostedtoolcache/Python/3.13.15/x64/lib/python3.13/site-packages/hdmf/spec/namespace.py:620: UserWarning: Ignoring the following cached namespace(s) because another version is already loaded:
core - cached version: 2.7.0, loaded version: 2.8.0
The loaded extension(s) may not be compatible with the cached extension(s) in the file. Please check the extension documentation and ignore this warning if these versions are compatible.
  self.warn_for_ignored_namespaces(ignored_namespaces)

nwbfile.subject

one_photon_series = nwbfile.acquisition["OnePhotonSeriesGreen"]
one_photon_series

nwbfile.imaging_planes["ImagingPlaneGreen"]

# Visualize the imaging data.

from matplotlib import pyplot as plt

frame = one_photon_series.data[50]
plt.imshow(frame)
plt.title("Raw Fiber Array Imaging")
plt.show()

fiber_photometry_response_series = nwbfile.acquisition["FiberPhotometryResponseSeriesGreen"]

import pandas as pd
from matplotlib import pyplot as plt

# Prepare data for plotting
fiber_indices = [0, 4]
data = fiber_photometry_response_series.data[100:500, fiber_indices]
timestamps = fiber_photometry_response_series.get_timestamps()[100:500]

fig, axes = plt.subplots(nrows=data.shape[1], ncols=1, figsize=(8, 3), sharey=True, sharex=True, dpi=300)

for i, ax in enumerate(axes):
    ax.plot(timestamps, data[:, i], linewidth=0.5, color="green")

    ax.tick_params(axis='y', labelsize=8)
    ax.tick_params(axis='x', labelsize=8)

    ax.legend([f"Fiber {i+1}"], frameon=False, bbox_to_anchor=(.95, 1), loc='upper left', prop={'size': 8})

    ax.spines['top'].set_visible(False)
    ax.spines['right'].set_visible(False)

axes[0].spines['bottom'].set_visible(False)
axes[0].set_title("Raw fluorescence traces", fontsize=8)
plt.xlabel('Time (s)', fontsize=8)
plt.tick_params(axis='x', labelsize=8)

plt.tight_layout()
plt.show()

nwbfile.lab_meta_data["FiberPhotometry"].fiber_photometry_table[:]

fiber_photometry_table_region = nwbfile.acquisition["FiberPhotometryResponseSeriesGreen"].fiber_photometry_table_region[:]
fiber_photometry_table_region.head()

fiber_photometry_table_region["optical_fiber"][0]

fiber_photometry_table_region["indicator"][0]

fiber_photometry_table_region["excitation_source"][0]

fiber_photometry_table_region["photodetector"][0]

fiber_photometry_table_region["dichroic_mirror"][0]

fiber_photometry_table_region["emission_filter"][0]

fiber_photometry_table_region["excitation_filter"][0]

nwbfile.processing["ophys"]

df_over_f_traces = nwbfile.processing["ophys"]["DfOverFFiberPhotometryResponseSeriesGreen"]

# Visualize the DF/F traces.
from matplotlib import pyplot as plt

# Prepare data for plotting
data = df_over_f_traces.data[100:500, :5]
timestamps = df_over_f_traces.get_timestamps()[100:500]

fig, axes = plt.subplots(nrows=data.shape[1], ncols=1, figsize=(6, 4), sharey=True, sharex=True, dpi=300)

for i, ax in enumerate(axes):
    ax.plot(timestamps, data[:, i], linewidth=0.5, color="green")

    ax.tick_params(axis='y', labelsize=6)
    ax.tick_params(axis='x', labelsize=6)

    ax.legend([f"∆F/F Fiber {i+1}"], frameon=False, bbox_to_anchor=(.95, 1), loc='upper left', prop={'size': 6})

    ax.spines['top'].set_visible(False)
    ax.spines['right'].set_visible(False)
    if i != data.shape[1] - 1:
        ax.spines['bottom'].set_visible(False)

axes[0].set_title("DF/F Fluorescence traces", fontsize=6)
plt.xlabel('Time (s)', fontsize=6)
plt.tick_params(axis='x', labelsize=6)

plt.tight_layout()
plt.show()

motion_corrected = nwbfile.processing["ophys"]["OnePhotonSeriesMotionCorrectedGreen"]
motion_corrected

# Visualize the motion corrected imaging data.

from matplotlib import pyplot as plt

fig, axes = plt.subplots(nrows=1, ncols=2, sharex=True, sharey=True, dpi=300)

axes[0].imshow(one_photon_series.data[50])
axes[0].set_title("Raw Fiber Array Imaging", fontsize=6)
axes[0].tick_params(axis='x', labelsize=6)
axes[0].tick_params(axis='y', labelsize=6)

axes[1].imshow(motion_corrected.data[50])
axes[1].set_title("Motion Corrected Fiber Array Imaging", fontsize=6)
axes[1].tick_params(axis='x', labelsize=6)
axes[1].tick_params(axis='y', labelsize=6)

plt.tight_layout()
plt.show()

rois_table = nwbfile.processing["ophys"]["ImageSegmentation"]["PlaneSegmentation"][:]
rois_table.head()

# Visualize the ROIs and the motion corrected imaging data.
from matplotlib import pyplot as plt
import numpy as np

plt.imshow(motion_corrected.data[50])

roi_masks = rois_table["image_mask"].values
roi_masks_combined = np.zeros(roi_masks[0].shape)
for i in range(roi_masks.shape[0]):
    inds = np.where(roi_masks[i] != 0)
    roi_masks_combined[inds] = 1

masked_roi_masks_combined = np.ma.masked_where(roi_masks_combined == 0, roi_masks_combined)
plt.imshow(masked_roi_masks_combined, cmap='gray', interpolation='none', alpha=0.5)
plt.title("ROIs on motion corrected image")
plt.show()

nwbfile.processing["behavior"]

velocity = nwbfile.processing["behavior"]["Velocity"]

# Visualize the DF/F traces and velocity for the roll in m/s.

import pandas as pd
from matplotlib import pyplot as plt

# Prepare data for plotting
data = df_over_f_traces.data[200:600, :2]
timestamps = df_over_f_traces.get_timestamps()[200:600]

velocity_x = velocity.data[200:600, 0]

fig, axes = plt.subplots(nrows=3, ncols=1, figsize=(6, 4), sharey=False, sharex=True, dpi=300)

for i in range(len(axes)-1):
    axes[i].plot(timestamps, data[:, i], linewidth=0.5, color="green")
    axes[i].spines['top'].set_visible(False)
    axes[i].spines['right'].set_visible(False)
    
    axes[i].tick_params(axis='y', labelsize=6)
    axes[i].tick_params(axis='x', labelsize=6)
    axes[i].spines['bottom'].set_visible(False)
    axes[i].set_ylim([-0.01, 0.09])

    axes[i].legend([f"∆F/F Fiber {i+1}"], frameon=False, bbox_to_anchor=(.95, 1), loc='upper left', prop={'size': 6})

    axes[i].spines['top'].set_visible(False)
    axes[i].spines['right'].set_visible(False)
            
axes[-1].plot(timestamps, velocity_x, color="black", alpha=0.8, linewidth=0.5)
axes[-1].spines['top'].set_visible(False)
axes[-1].spines['right'].set_visible(False)
axes[-1].legend(["Roll velocity"], frameon=False, bbox_to_anchor=(.95, 1), loc='upper left', prop={'size': 6})
axes[-1].tick_params(axis='y', labelsize=6)
axes[-1].tick_params(axis='x', labelsize=6)

plt.xlabel('Time (s)', fontsize=6)
plt.tight_layout()
plt.show()

events = nwbfile.processing["behavior"]["TimeIntervals"][:]
events

from matplotlib import pyplot as plt
import matplotlib.lines as mlines

# Prepare data for plotting
data = df_over_f_traces.data[700:1200, 0]
timestamps = df_over_f_traces.get_timestamps()[700:1200]

fig, ax = plt.subplots(nrows=1, ncols=1, figsize=(6, 2), dpi=300, sharex=True)

light_events = events[events["event_type"] == "Light"]
light_events = light_events[(light_events["start_time"] >= timestamps[0]) & (light_events["stop_time"] < timestamps[-1])]

tone_events = events[events["event_type"] == "Tone"]
tone_events = tone_events[(tone_events["start_time"] >= timestamps[0]) & (tone_events["stop_time"] < timestamps[-1])]

ax.plot(timestamps, data, color="green", linewidth=0.5)

for ind, row in light_events.iterrows():
    ax.fill_between(timestamps, min(data), max(data), where=(timestamps >= row["start_time"]) & (timestamps <= row["stop_time"]), color='blue', edgecolor='none', alpha=0.1)

for ind, row in tone_events.iterrows():
    ax.fill_between(timestamps, min(data), max(data), where=(timestamps >= row["start_time"]) & (timestamps <= row["stop_time"]), color='red', edgecolor='none', alpha=0.1)

# Create proxy lines for legend entries with corresponding colors and transparency
green_line = mlines.Line2D([], [], color='green', label='∆F/F from striatum', alpha=0.5)
blue_line = mlines.Line2D([], [], color='blue', label='Light', alpha=0.1)
red_line = mlines.Line2D([], [], color='red', label='Tone', alpha=0.1)

ax.legend(handles=[green_line, blue_line, red_line], frameon=False, bbox_to_anchor=(.95, 1), loc='upper left', prop={'size': 6})

ax.spines['top'].set_visible(False)
ax.spines['right'].set_visible(False)
ax.tick_params(axis='y', labelsize=6)
ax.tick_params(axis='x', labelsize=6)
plt.xlabel('Time (s)', fontsize=6)

plt.show()

Data type	uint16
Shape	(40000, 376, 375)
Array size	10.51 GiB
Chunk shape	(35, 376, 375)
Compression	gzip
Compression opts	4
Uncompressed size (bytes)	11280000000
Compressed size (bytes)	9213518925
Compression ratio	1.2242879286211483

Data type	int64
Shape	(2,)
Array size	16.00 bytes
Chunk shape	None
Compression	None
Compression opts	None
Uncompressed size (bytes)	16
Compressed size (bytes)	16
Compression ratio	1.0

	location	indicator	optical_fiber	excitation_source	photodetector	dichroic_mirror	allen_atlas_coordinates	included	coordinates	emission_filter	excitation_filter
id
0	Caudoputamen	dLight1.3b abc.Indicator at 0x140603529377984\...	FiberArray abc.OpticalFiber at 0x1406035293773...	ExcitationSource470 abc.ExcitationSource at 0x...	CMOSCamera pynwb.device.Device at 0x1406035301...	DichroicMirror1 abc.DichroicMirror at 0x140603...	[442, 644, 229]	False	[0.83, 0.74, 1.78]	OpticalFilter525 abc.BandOpticalFilter at 0x14...	OpticalFilter470 abc.BandOpticalFilter at 0x14...
1	Caudoputamen	dLight1.3b abc.Indicator at 0x140603529377984\...	FiberArray abc.OpticalFiber at 0x1406035293773...	ExcitationSource470 abc.ExcitationSource at 0x...	CMOSCamera pynwb.device.Device at 0x1406035301...	DichroicMirror1 abc.DichroicMirror at 0x140603...	[454, 642, 304]	False	[0.71, 0.72, 2.44]	OpticalFilter525 abc.BandOpticalFilter at 0x14...	OpticalFilter470 abc.BandOpticalFilter at 0x14...
2	Primary motor area Layer 6a	dLight1.3b abc.Indicator at 0x140603529377984\...	FiberArray abc.OpticalFiber at 0x1406035293773...	ExcitationSource470 abc.ExcitationSource at 0x...	CMOSCamera pynwb.device.Device at 0x1406035301...	DichroicMirror1 abc.DichroicMirror at 0x140603...	[460, 674, 180]	False	[0.65, 1.04, 1.35]	OpticalFilter525 abc.BandOpticalFilter at 0x14...	OpticalFilter470 abc.BandOpticalFilter at 0x14...
3	Caudoputamen	dLight1.3b abc.Indicator at 0x140603529377984\...	FiberArray abc.OpticalFiber at 0x1406035293773...	ExcitationSource470 abc.ExcitationSource at 0x...	CMOSCamera pynwb.device.Device at 0x1406035301...	DichroicMirror1 abc.DichroicMirror at 0x140603...	[495, 685, 260]	False	[0.3, 1.15, 2.05]	OpticalFilter525 abc.BandOpticalFilter at 0x14...	OpticalFilter470 abc.BandOpticalFilter at 0x14...
4	Primary motor area Layer 6a	dLight1.3b abc.Indicator at 0x140603529377984\...	FiberArray abc.OpticalFiber at 0x1406035293773...	ExcitationSource470 abc.ExcitationSource at 0x...	CMOSCamera pynwb.device.Device at 0x1406035301...	DichroicMirror1 abc.DichroicMirror at 0x140603...	[400, 686, 160]	False	[1.25, 1.16, 1.17]	OpticalFilter525 abc.BandOpticalFilter at 0x14...	OpticalFilter470 abc.BandOpticalFilter at 0x14...
...	...	...	...	...	...	...	...	...	...	...	...
98	Caudoputamen	dLight1.3b abc.Indicator at 0x140603529377984\...	FiberArray abc.OpticalFiber at 0x1406035293773...	ExcitationSource470 abc.ExcitationSource at 0x...	CMOSCamera pynwb.device.Device at 0x1406035301...	DichroicMirror1 abc.DichroicMirror at 0x140603...	[433, 775, 249]	False	[0.92, 2.05, 1.96]	OpticalFilter525 abc.BandOpticalFilter at 0x14...	OpticalFilter470 abc.BandOpticalFilter at 0x14...
99	Caudoputamen	dLight1.3b abc.Indicator at 0x140603529377984\...	FiberArray abc.OpticalFiber at 0x1406035293773...	ExcitationSource470 abc.ExcitationSource at 0x...	CMOSCamera pynwb.device.Device at 0x1406035301...	DichroicMirror1 abc.DichroicMirror at 0x140603...	[444, 815, 381]	True	[0.81, 2.45, 3.12]	OpticalFilter525 abc.BandOpticalFilter at 0x14...	OpticalFilter470 abc.BandOpticalFilter at 0x14...
100	Caudoputamen	dLight1.3b abc.Indicator at 0x140603529377984\...	FiberArray abc.OpticalFiber at 0x1406035293773...	ExcitationSource470 abc.ExcitationSource at 0x...	CMOSCamera pynwb.device.Device at 0x1406035301...	DichroicMirror1 abc.DichroicMirror at 0x140603...	[457, 833, 280]	False	[0.68, 2.63, 2.23]	OpticalFilter525 abc.BandOpticalFilter at 0x14...	OpticalFilter470 abc.BandOpticalFilter at 0x14...
101	Primary somatosensory area mouth layer 6a	dLight1.3b abc.Indicator at 0x140603529377984\...	FiberArray abc.OpticalFiber at 0x1406035293773...	ExcitationSource470 abc.ExcitationSource at 0x...	CMOSCamera pynwb.device.Device at 0x1406035301...	DichroicMirror1 abc.DichroicMirror at 0x140603...	[482, 821, 396]	False	[0.43, 2.51, 3.25]	OpticalFilter525 abc.BandOpticalFilter at 0x14...	OpticalFilter470 abc.BandOpticalFilter at 0x14...
102	Primary motor area Layer 2/3	dLight1.3b abc.Indicator at 0x140603529377984\...	FiberArray abc.OpticalFiber at 0x1406035293773...	ExcitationSource470 abc.ExcitationSource at 0x...	CMOSCamera pynwb.device.Device at 0x1406035301...	DichroicMirror1 abc.DichroicMirror at 0x140603...	[396, 791, 209]	False	[1.29, 2.21, 1.6]	OpticalFilter525 abc.BandOpticalFilter at 0x14...	OpticalFilter470 abc.BandOpticalFilter at 0x14...

	location	indicator	optical_fiber	excitation_source	photodetector	dichroic_mirror	allen_atlas_coordinates	included	coordinates	emission_filter	excitation_filter
id
0	Caudoputamen	dLight1.3b abc.Indicator at 0x140603529377984\...	FiberArray abc.OpticalFiber at 0x1406035293773...	ExcitationSource470 abc.ExcitationSource at 0x...	CMOSCamera pynwb.device.Device at 0x1406035301...	DichroicMirror1 abc.DichroicMirror at 0x140603...	[442, 644, 229]	False	[0.83, 0.74, 1.78]	OpticalFilter525 abc.BandOpticalFilter at 0x14...	OpticalFilter470 abc.BandOpticalFilter at 0x14...
1	Caudoputamen	dLight1.3b abc.Indicator at 0x140603529377984\...	FiberArray abc.OpticalFiber at 0x1406035293773...	ExcitationSource470 abc.ExcitationSource at 0x...	CMOSCamera pynwb.device.Device at 0x1406035301...	DichroicMirror1 abc.DichroicMirror at 0x140603...	[454, 642, 304]	False	[0.71, 0.72, 2.44]	OpticalFilter525 abc.BandOpticalFilter at 0x14...	OpticalFilter470 abc.BandOpticalFilter at 0x14...
2	Primary motor area Layer 6a	dLight1.3b abc.Indicator at 0x140603529377984\...	FiberArray abc.OpticalFiber at 0x1406035293773...	ExcitationSource470 abc.ExcitationSource at 0x...	CMOSCamera pynwb.device.Device at 0x1406035301...	DichroicMirror1 abc.DichroicMirror at 0x140603...	[460, 674, 180]	False	[0.65, 1.04, 1.35]	OpticalFilter525 abc.BandOpticalFilter at 0x14...	OpticalFilter470 abc.BandOpticalFilter at 0x14...
3	Caudoputamen	dLight1.3b abc.Indicator at 0x140603529377984\...	FiberArray abc.OpticalFiber at 0x1406035293773...	ExcitationSource470 abc.ExcitationSource at 0x...	CMOSCamera pynwb.device.Device at 0x1406035301...	DichroicMirror1 abc.DichroicMirror at 0x140603...	[495, 685, 260]	False	[0.3, 1.15, 2.05]	OpticalFilter525 abc.BandOpticalFilter at 0x14...	OpticalFilter470 abc.BandOpticalFilter at 0x14...
4	Primary motor area Layer 6a	dLight1.3b abc.Indicator at 0x140603529377984\...	FiberArray abc.OpticalFiber at 0x1406035293773...	ExcitationSource470 abc.ExcitationSource at 0x...	CMOSCamera pynwb.device.Device at 0x1406035301...	DichroicMirror1 abc.DichroicMirror at 0x140603...	[400, 686, 160]	False	[1.25, 1.16, 1.17]	OpticalFilter525 abc.BandOpticalFilter at 0x14...	OpticalFilter470 abc.BandOpticalFilter at 0x14...

Data type	float64
Shape	(2,)
Array size	16.00 bytes

Installing requirements¶

Vu 2024 demo¶

Streaming an NWB file¶

Access subject metadata¶

subject (Subject)

Access raw imaging¶

Fiber array imaging¶

OnePhotonSeriesGreen (OnePhotonSeries)

ImagingPlaneGreen (ImagingPlane)

Fiber photometry traces¶

Fiber photometry metadata¶

FiberArray (OpticalFiber)

dLight1.3b (Indicator)

ExcitationSource470 (ExcitationSource)

CMOSCamera (Device)

DichroicMirror1 (DichroicMirror)

OpticalFilter525 (BandOpticalFilter)

OpticalFilter470 (BandOpticalFilter)

Access processed fiber photometry data¶

ophys (ProcessingModule)

Access motion corrected imaging data¶

OnePhotonSeriesMotionCorrectedGreen (OnePhotonSeries)

Access ROIs¶

Access behavior data¶

behavior (ProcessingModule)

	image_mask	ROICentroids	Accepted	Rejected
id
0	[[0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0,...	[166.47541258012367, 59.00562445763765]	0	1
1	[[0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0,...	[163.1369152701555, 80.29428559091824]	0	1
2	[[0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0,...	[144.14977970147507, 83.10331721340896]	0	1
3	[[0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0,...	[123.4401911607665, 85.54001655461578]	0	1
4	[[0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0,...	[111.00957492596739, 100.35934701854168]	0	1

	start_time	stop_time	event_type
id
0	10.21300	11.08000	Light
1	25.31550	26.18225	Light
2	36.25050	37.58400	Tone
3	69.78875	70.65550	Light

	location	indicator	optical_fiber	excitation_source	photodetector	dichroic_mirror	allen_atlas_coordinates	included	coordinates	emission_filter	excitation_filter
id
0	Caudoputamen	dLight1.3b abc.Indicator at 0x140603529377984\nFields:\n description: green dopamine sensor\n label: pAAV-CAG-dLight1.3b(AAV5)\n	FiberArray abc.OpticalFiber at 0x140603529377312\nFields:\n core_diameter_in_um: 34.0\n description: The optical fiber used in a multi-fiber arrays configuration, fabricated in-house to enable large scale measurements across deep brain volumes. Bare fibers were cut into pieces (ca. 3cm) then mounted under a microscope into 55-60μm diameter holes in a custom 3D printed grid (3mm W x 5mm L, Boston Micro Fabrication), measured under a dissection microscope to target a particular depth beneath the grid, and secured in place with UV glue (Norland Optical Adhesive 61). Each array contained between 30 and 103 fibers separated by a minimum of 220μm radially and 250μm axially. Separation was calculated to achieve maximal coverage of the striatum volume with no overlap in the collection fields of individual fibers. Fibers were cut with a fiber scribe, and the distal ends were inspected to ensure a uniform cut, and re-cut as necessary. Distal ends were then glued inside an 1cm ca. section of polyimide tube (0.8-1.3mm diameter, MicroLumen) then cut with a fresh razorblade. The bundled fibers inside the tube were then polished on fine grained polishing paper (ThorLabs,polished first with 6 μm, followed by 3 μm) to create a smooth, uniform fiber bundle surface for imaging. A larger diameter post was mounted on one side of the plastic grid to facilitate holding during implantation.\n manufacturer: Fiber Optics Tech\n model: not specified\n numerical_aperture: 0.66\n	ExcitationSource470 abc.ExcitationSource at 0x140603529376976\nFields:\n description: Blue excitation light (470 nm LED, Thorlabs, No. SOLIS-470C) and violet excitation light (for the isosbestic control)\nwere coupled into the optic fiber such that a power of 0.75 mW was delivered to the fiber tip.\nThen, 470 nm and 405 nm excitation were alternated at 100 Hz using a waveform generator,\neach filtered with a corresponding filter.\n\n excitation_wavelength_in_nm: 470.0\n illumination_type: LED\n manufacturer: Thorlabs\n model: SOLIS-470C\n	CMOSCamera pynwb.device.Device at 0x140603530107856\nFields:\n description: A tube lens in each path (Thor labs, No TTL165-A) focused emission light onto the CMOS sensors of the cameras to form an image of the fiber bundle.\n manufacturer: Hamamatsu Photonics, Orca Fusion BT Gen III\n	DichroicMirror1 abc.DichroicMirror at 0x140603529376640\nFields:\n cut_on_wavelength_in_nm: 532.0\n description: The dichroic mirror used to reflect green and pass red fluorescence\n manufacturer: Chroma Tech Corp\n model: ZT532rdc\n reflection_band_in_nm: [405. 532.]\n transmission_band_in_nm: [545. 750.]\n	[442, 644, 229]	False	[0.83, 0.74, 1.78]	OpticalFilter525 abc.BandOpticalFilter at 0x140603530113296\nFields:\n bandwidth_in_nm: 50.0\n center_wavelength_in_nm: 525.0\n description: The band-pass filter used to isolate the green emitted light after passing through a dichroic (Chroma, No. 532rdc) that reflected green and passed red fluorescence.\n filter_type: Bandpass\n manufacturer: Chroma\n model: 525/50m\n	OpticalFilter470 abc.BandOpticalFilter at 0x140603529377648\nFields:\n bandwidth_in_nm: 24.0\n center_wavelength_in_nm: 473.0\n description: The band-pass filter used to isolate the 470 nm excitation light.\n filter_type: Bandpass\n manufacturer: Chroma\n model: ET473/24\n
1	Caudoputamen	dLight1.3b abc.Indicator at 0x140603529377984\nFields:\n description: green dopamine sensor\n label: pAAV-CAG-dLight1.3b(AAV5)\n	FiberArray abc.OpticalFiber at 0x140603529377312\nFields:\n core_diameter_in_um: 34.0\n description: The optical fiber used in a multi-fiber arrays configuration, fabricated in-house to enable large scale measurements across deep brain volumes. Bare fibers were cut into pieces (ca. 3cm) then mounted under a microscope into 55-60μm diameter holes in a custom 3D printed grid (3mm W x 5mm L, Boston Micro Fabrication), measured under a dissection microscope to target a particular depth beneath the grid, and secured in place with UV glue (Norland Optical Adhesive 61). Each array contained between 30 and 103 fibers separated by a minimum of 220μm radially and 250μm axially. Separation was calculated to achieve maximal coverage of the striatum volume with no overlap in the collection fields of individual fibers. Fibers were cut with a fiber scribe, and the distal ends were inspected to ensure a uniform cut, and re-cut as necessary. Distal ends were then glued inside an 1cm ca. section of polyimide tube (0.8-1.3mm diameter, MicroLumen) then cut with a fresh razorblade. The bundled fibers inside the tube were then polished on fine grained polishing paper (ThorLabs,polished first with 6 μm, followed by 3 μm) to create a smooth, uniform fiber bundle surface for imaging. A larger diameter post was mounted on one side of the plastic grid to facilitate holding during implantation.\n manufacturer: Fiber Optics Tech\n model: not specified\n numerical_aperture: 0.66\n	ExcitationSource470 abc.ExcitationSource at 0x140603529376976\nFields:\n description: Blue excitation light (470 nm LED, Thorlabs, No. SOLIS-470C) and violet excitation light (for the isosbestic control)\nwere coupled into the optic fiber such that a power of 0.75 mW was delivered to the fiber tip.\nThen, 470 nm and 405 nm excitation were alternated at 100 Hz using a waveform generator,\neach filtered with a corresponding filter.\n\n excitation_wavelength_in_nm: 470.0\n illumination_type: LED\n manufacturer: Thorlabs\n model: SOLIS-470C\n	CMOSCamera pynwb.device.Device at 0x140603530107856\nFields:\n description: A tube lens in each path (Thor labs, No TTL165-A) focused emission light onto the CMOS sensors of the cameras to form an image of the fiber bundle.\n manufacturer: Hamamatsu Photonics, Orca Fusion BT Gen III\n	DichroicMirror1 abc.DichroicMirror at 0x140603529376640\nFields:\n cut_on_wavelength_in_nm: 532.0\n description: The dichroic mirror used to reflect green and pass red fluorescence\n manufacturer: Chroma Tech Corp\n model: ZT532rdc\n reflection_band_in_nm: [405. 532.]\n transmission_band_in_nm: [545. 750.]\n	[454, 642, 304]	False	[0.71, 0.72, 2.44]	OpticalFilter525 abc.BandOpticalFilter at 0x140603530113296\nFields:\n bandwidth_in_nm: 50.0\n center_wavelength_in_nm: 525.0\n description: The band-pass filter used to isolate the green emitted light after passing through a dichroic (Chroma, No. 532rdc) that reflected green and passed red fluorescence.\n filter_type: Bandpass\n manufacturer: Chroma\n model: 525/50m\n	OpticalFilter470 abc.BandOpticalFilter at 0x140603529377648\nFields:\n bandwidth_in_nm: 24.0\n center_wavelength_in_nm: 473.0\n description: The band-pass filter used to isolate the 470 nm excitation light.\n filter_type: Bandpass\n manufacturer: Chroma\n model: ET473/24\n
2	Primary motor area Layer 6a	dLight1.3b abc.Indicator at 0x140603529377984\nFields:\n description: green dopamine sensor\n label: pAAV-CAG-dLight1.3b(AAV5)\n	FiberArray abc.OpticalFiber at 0x140603529377312\nFields:\n core_diameter_in_um: 34.0\n description: The optical fiber used in a multi-fiber arrays configuration, fabricated in-house to enable large scale measurements across deep brain volumes. Bare fibers were cut into pieces (ca. 3cm) then mounted under a microscope into 55-60μm diameter holes in a custom 3D printed grid (3mm W x 5mm L, Boston Micro Fabrication), measured under a dissection microscope to target a particular depth beneath the grid, and secured in place with UV glue (Norland Optical Adhesive 61). Each array contained between 30 and 103 fibers separated by a minimum of 220μm radially and 250μm axially. Separation was calculated to achieve maximal coverage of the striatum volume with no overlap in the collection fields of individual fibers. Fibers were cut with a fiber scribe, and the distal ends were inspected to ensure a uniform cut, and re-cut as necessary. Distal ends were then glued inside an 1cm ca. section of polyimide tube (0.8-1.3mm diameter, MicroLumen) then cut with a fresh razorblade. The bundled fibers inside the tube were then polished on fine grained polishing paper (ThorLabs,polished first with 6 μm, followed by 3 μm) to create a smooth, uniform fiber bundle surface for imaging. A larger diameter post was mounted on one side of the plastic grid to facilitate holding during implantation.\n manufacturer: Fiber Optics Tech\n model: not specified\n numerical_aperture: 0.66\n	ExcitationSource470 abc.ExcitationSource at 0x140603529376976\nFields:\n description: Blue excitation light (470 nm LED, Thorlabs, No. SOLIS-470C) and violet excitation light (for the isosbestic control)\nwere coupled into the optic fiber such that a power of 0.75 mW was delivered to the fiber tip.\nThen, 470 nm and 405 nm excitation were alternated at 100 Hz using a waveform generator,\neach filtered with a corresponding filter.\n\n excitation_wavelength_in_nm: 470.0\n illumination_type: LED\n manufacturer: Thorlabs\n model: SOLIS-470C\n	CMOSCamera pynwb.device.Device at 0x140603530107856\nFields:\n description: A tube lens in each path (Thor labs, No TTL165-A) focused emission light onto the CMOS sensors of the cameras to form an image of the fiber bundle.\n manufacturer: Hamamatsu Photonics, Orca Fusion BT Gen III\n	DichroicMirror1 abc.DichroicMirror at 0x140603529376640\nFields:\n cut_on_wavelength_in_nm: 532.0\n description: The dichroic mirror used to reflect green and pass red fluorescence\n manufacturer: Chroma Tech Corp\n model: ZT532rdc\n reflection_band_in_nm: [405. 532.]\n transmission_band_in_nm: [545. 750.]\n	[460, 674, 180]	False	[0.65, 1.04, 1.35]	OpticalFilter525 abc.BandOpticalFilter at 0x140603530113296\nFields:\n bandwidth_in_nm: 50.0\n center_wavelength_in_nm: 525.0\n description: The band-pass filter used to isolate the green emitted light after passing through a dichroic (Chroma, No. 532rdc) that reflected green and passed red fluorescence.\n filter_type: Bandpass\n manufacturer: Chroma\n model: 525/50m\n	OpticalFilter470 abc.BandOpticalFilter at 0x140603529377648\nFields:\n bandwidth_in_nm: 24.0\n center_wavelength_in_nm: 473.0\n description: The band-pass filter used to isolate the 470 nm excitation light.\n filter_type: Bandpass\n manufacturer: Chroma\n model: ET473/24\n
3	Caudoputamen	dLight1.3b abc.Indicator at 0x140603529377984\nFields:\n description: green dopamine sensor\n label: pAAV-CAG-dLight1.3b(AAV5)\n	FiberArray abc.OpticalFiber at 0x140603529377312\nFields:\n core_diameter_in_um: 34.0\n description: The optical fiber used in a multi-fiber arrays configuration, fabricated in-house to enable large scale measurements across deep brain volumes. Bare fibers were cut into pieces (ca. 3cm) then mounted under a microscope into 55-60μm diameter holes in a custom 3D printed grid (3mm W x 5mm L, Boston Micro Fabrication), measured under a dissection microscope to target a particular depth beneath the grid, and secured in place with UV glue (Norland Optical Adhesive 61). Each array contained between 30 and 103 fibers separated by a minimum of 220μm radially and 250μm axially. Separation was calculated to achieve maximal coverage of the striatum volume with no overlap in the collection fields of individual fibers. Fibers were cut with a fiber scribe, and the distal ends were inspected to ensure a uniform cut, and re-cut as necessary. Distal ends were then glued inside an 1cm ca. section of polyimide tube (0.8-1.3mm diameter, MicroLumen) then cut with a fresh razorblade. The bundled fibers inside the tube were then polished on fine grained polishing paper (ThorLabs,polished first with 6 μm, followed by 3 μm) to create a smooth, uniform fiber bundle surface for imaging. A larger diameter post was mounted on one side of the plastic grid to facilitate holding during implantation.\n manufacturer: Fiber Optics Tech\n model: not specified\n numerical_aperture: 0.66\n	ExcitationSource470 abc.ExcitationSource at 0x140603529376976\nFields:\n description: Blue excitation light (470 nm LED, Thorlabs, No. SOLIS-470C) and violet excitation light (for the isosbestic control)\nwere coupled into the optic fiber such that a power of 0.75 mW was delivered to the fiber tip.\nThen, 470 nm and 405 nm excitation were alternated at 100 Hz using a waveform generator,\neach filtered with a corresponding filter.\n\n excitation_wavelength_in_nm: 470.0\n illumination_type: LED\n manufacturer: Thorlabs\n model: SOLIS-470C\n	CMOSCamera pynwb.device.Device at 0x140603530107856\nFields:\n description: A tube lens in each path (Thor labs, No TTL165-A) focused emission light onto the CMOS sensors of the cameras to form an image of the fiber bundle.\n manufacturer: Hamamatsu Photonics, Orca Fusion BT Gen III\n	DichroicMirror1 abc.DichroicMirror at 0x140603529376640\nFields:\n cut_on_wavelength_in_nm: 532.0\n description: The dichroic mirror used to reflect green and pass red fluorescence\n manufacturer: Chroma Tech Corp\n model: ZT532rdc\n reflection_band_in_nm: [405. 532.]\n transmission_band_in_nm: [545. 750.]\n	[495, 685, 260]	False	[0.3, 1.15, 2.05]	OpticalFilter525 abc.BandOpticalFilter at 0x140603530113296\nFields:\n bandwidth_in_nm: 50.0\n center_wavelength_in_nm: 525.0\n description: The band-pass filter used to isolate the green emitted light after passing through a dichroic (Chroma, No. 532rdc) that reflected green and passed red fluorescence.\n filter_type: Bandpass\n manufacturer: Chroma\n model: 525/50m\n	OpticalFilter470 abc.BandOpticalFilter at 0x140603529377648\nFields:\n bandwidth_in_nm: 24.0\n center_wavelength_in_nm: 473.0\n description: The band-pass filter used to isolate the 470 nm excitation light.\n filter_type: Bandpass\n manufacturer: Chroma\n model: ET473/24\n

	location	indicator	optical_fiber	excitation_source	photodetector	dichroic_mirror	allen_atlas_coordinates	included	coordinates	emission_filter	excitation_filter
id
0	Caudoputamen	dLight1.3b abc.Indicator at 0x140603529377984\nFields:\n description: green dopamine sensor\n label: pAAV-CAG-dLight1.3b(AAV5)\n	FiberArray abc.OpticalFiber at 0x140603529377312\nFields:\n core_diameter_in_um: 34.0\n description: The optical fiber used in a multi-fiber arrays configuration, fabricated in-house to enable large scale measurements across deep brain volumes. Bare fibers were cut into pieces (ca. 3cm) then mounted under a microscope into 55-60μm diameter holes in a custom 3D printed grid (3mm W x 5mm L, Boston Micro Fabrication), measured under a dissection microscope to target a particular depth beneath the grid, and secured in place with UV glue (Norland Optical Adhesive 61). Each array contained between 30 and 103 fibers separated by a minimum of 220μm radially and 250μm axially. Separation was calculated to achieve maximal coverage of the striatum volume with no overlap in the collection fields of individual fibers. Fibers were cut with a fiber scribe, and the distal ends were inspected to ensure a uniform cut, and re-cut as necessary. Distal ends were then glued inside an 1cm ca. section of polyimide tube (0.8-1.3mm diameter, MicroLumen) then cut with a fresh razorblade. The bundled fibers inside the tube were then polished on fine grained polishing paper (ThorLabs,polished first with 6 μm, followed by 3 μm) to create a smooth, uniform fiber bundle surface for imaging. A larger diameter post was mounted on one side of the plastic grid to facilitate holding during implantation.\n manufacturer: Fiber Optics Tech\n model: not specified\n numerical_aperture: 0.66\n	ExcitationSource470 abc.ExcitationSource at 0x140603529376976\nFields:\n description: Blue excitation light (470 nm LED, Thorlabs, No. SOLIS-470C) and violet excitation light (for the isosbestic control)\nwere coupled into the optic fiber such that a power of 0.75 mW was delivered to the fiber tip.\nThen, 470 nm and 405 nm excitation were alternated at 100 Hz using a waveform generator,\neach filtered with a corresponding filter.\n\n excitation_wavelength_in_nm: 470.0\n illumination_type: LED\n manufacturer: Thorlabs\n model: SOLIS-470C\n	CMOSCamera pynwb.device.Device at 0x140603530107856\nFields:\n description: A tube lens in each path (Thor labs, No TTL165-A) focused emission light onto the CMOS sensors of the cameras to form an image of the fiber bundle.\n manufacturer: Hamamatsu Photonics, Orca Fusion BT Gen III\n	DichroicMirror1 abc.DichroicMirror at 0x140603529376640\nFields:\n cut_on_wavelength_in_nm: 532.0\n description: The dichroic mirror used to reflect green and pass red fluorescence\n manufacturer: Chroma Tech Corp\n model: ZT532rdc\n reflection_band_in_nm: [405. 532.]\n transmission_band_in_nm: [545. 750.]\n	[442, 644, 229]	False	[0.83, 0.74, 1.78]	OpticalFilter525 abc.BandOpticalFilter at 0x140603530113296\nFields:\n bandwidth_in_nm: 50.0\n center_wavelength_in_nm: 525.0\n description: The band-pass filter used to isolate the green emitted light after passing through a dichroic (Chroma, No. 532rdc) that reflected green and passed red fluorescence.\n filter_type: Bandpass\n manufacturer: Chroma\n model: 525/50m\n	OpticalFilter470 abc.BandOpticalFilter at 0x140603529377648\nFields:\n bandwidth_in_nm: 24.0\n center_wavelength_in_nm: 473.0\n description: The band-pass filter used to isolate the 470 nm excitation light.\n filter_type: Bandpass\n manufacturer: Chroma\n model: ET473/24\n
1	Caudoputamen	dLight1.3b abc.Indicator at 0x140603529377984\nFields:\n description: green dopamine sensor\n label: pAAV-CAG-dLight1.3b(AAV5)\n	FiberArray abc.OpticalFiber at 0x140603529377312\nFields:\n core_diameter_in_um: 34.0\n description: The optical fiber used in a multi-fiber arrays configuration, fabricated in-house to enable large scale measurements across deep brain volumes. Bare fibers were cut into pieces (ca. 3cm) then mounted under a microscope into 55-60μm diameter holes in a custom 3D printed grid (3mm W x 5mm L, Boston Micro Fabrication), measured under a dissection microscope to target a particular depth beneath the grid, and secured in place with UV glue (Norland Optical Adhesive 61). Each array contained between 30 and 103 fibers separated by a minimum of 220μm radially and 250μm axially. Separation was calculated to achieve maximal coverage of the striatum volume with no overlap in the collection fields of individual fibers. Fibers were cut with a fiber scribe, and the distal ends were inspected to ensure a uniform cut, and re-cut as necessary. Distal ends were then glued inside an 1cm ca. section of polyimide tube (0.8-1.3mm diameter, MicroLumen) then cut with a fresh razorblade. The bundled fibers inside the tube were then polished on fine grained polishing paper (ThorLabs,polished first with 6 μm, followed by 3 μm) to create a smooth, uniform fiber bundle surface for imaging. A larger diameter post was mounted on one side of the plastic grid to facilitate holding during implantation.\n manufacturer: Fiber Optics Tech\n model: not specified\n numerical_aperture: 0.66\n	ExcitationSource470 abc.ExcitationSource at 0x140603529376976\nFields:\n description: Blue excitation light (470 nm LED, Thorlabs, No. SOLIS-470C) and violet excitation light (for the isosbestic control)\nwere coupled into the optic fiber such that a power of 0.75 mW was delivered to the fiber tip.\nThen, 470 nm and 405 nm excitation were alternated at 100 Hz using a waveform generator,\neach filtered with a corresponding filter.\n\n excitation_wavelength_in_nm: 470.0\n illumination_type: LED\n manufacturer: Thorlabs\n model: SOLIS-470C\n	CMOSCamera pynwb.device.Device at 0x140603530107856\nFields:\n description: A tube lens in each path (Thor labs, No TTL165-A) focused emission light onto the CMOS sensors of the cameras to form an image of the fiber bundle.\n manufacturer: Hamamatsu Photonics, Orca Fusion BT Gen III\n	DichroicMirror1 abc.DichroicMirror at 0x140603529376640\nFields:\n cut_on_wavelength_in_nm: 532.0\n description: The dichroic mirror used to reflect green and pass red fluorescence\n manufacturer: Chroma Tech Corp\n model: ZT532rdc\n reflection_band_in_nm: [405. 532.]\n transmission_band_in_nm: [545. 750.]\n	[454, 642, 304]	False	[0.71, 0.72, 2.44]	OpticalFilter525 abc.BandOpticalFilter at 0x140603530113296\nFields:\n bandwidth_in_nm: 50.0\n center_wavelength_in_nm: 525.0\n description: The band-pass filter used to isolate the green emitted light after passing through a dichroic (Chroma, No. 532rdc) that reflected green and passed red fluorescence.\n filter_type: Bandpass\n manufacturer: Chroma\n model: 525/50m\n	OpticalFilter470 abc.BandOpticalFilter at 0x140603529377648\nFields:\n bandwidth_in_nm: 24.0\n center_wavelength_in_nm: 473.0\n description: The band-pass filter used to isolate the 470 nm excitation light.\n filter_type: Bandpass\n manufacturer: Chroma\n model: ET473/24\n
2	Primary motor area Layer 6a	dLight1.3b abc.Indicator at 0x140603529377984\nFields:\n description: green dopamine sensor\n label: pAAV-CAG-dLight1.3b(AAV5)\n	FiberArray abc.OpticalFiber at 0x140603529377312\nFields:\n core_diameter_in_um: 34.0\n description: The optical fiber used in a multi-fiber arrays configuration, fabricated in-house to enable large scale measurements across deep brain volumes. Bare fibers were cut into pieces (ca. 3cm) then mounted under a microscope into 55-60μm diameter holes in a custom 3D printed grid (3mm W x 5mm L, Boston Micro Fabrication), measured under a dissection microscope to target a particular depth beneath the grid, and secured in place with UV glue (Norland Optical Adhesive 61). Each array contained between 30 and 103 fibers separated by a minimum of 220μm radially and 250μm axially. Separation was calculated to achieve maximal coverage of the striatum volume with no overlap in the collection fields of individual fibers. Fibers were cut with a fiber scribe, and the distal ends were inspected to ensure a uniform cut, and re-cut as necessary. Distal ends were then glued inside an 1cm ca. section of polyimide tube (0.8-1.3mm diameter, MicroLumen) then cut with a fresh razorblade. The bundled fibers inside the tube were then polished on fine grained polishing paper (ThorLabs,polished first with 6 μm, followed by 3 μm) to create a smooth, uniform fiber bundle surface for imaging. A larger diameter post was mounted on one side of the plastic grid to facilitate holding during implantation.\n manufacturer: Fiber Optics Tech\n model: not specified\n numerical_aperture: 0.66\n	ExcitationSource470 abc.ExcitationSource at 0x140603529376976\nFields:\n description: Blue excitation light (470 nm LED, Thorlabs, No. SOLIS-470C) and violet excitation light (for the isosbestic control)\nwere coupled into the optic fiber such that a power of 0.75 mW was delivered to the fiber tip.\nThen, 470 nm and 405 nm excitation were alternated at 100 Hz using a waveform generator,\neach filtered with a corresponding filter.\n\n excitation_wavelength_in_nm: 470.0\n illumination_type: LED\n manufacturer: Thorlabs\n model: SOLIS-470C\n	CMOSCamera pynwb.device.Device at 0x140603530107856\nFields:\n description: A tube lens in each path (Thor labs, No TTL165-A) focused emission light onto the CMOS sensors of the cameras to form an image of the fiber bundle.\n manufacturer: Hamamatsu Photonics, Orca Fusion BT Gen III\n	DichroicMirror1 abc.DichroicMirror at 0x140603529376640\nFields:\n cut_on_wavelength_in_nm: 532.0\n description: The dichroic mirror used to reflect green and pass red fluorescence\n manufacturer: Chroma Tech Corp\n model: ZT532rdc\n reflection_band_in_nm: [405. 532.]\n transmission_band_in_nm: [545. 750.]\n	[460, 674, 180]	False	[0.65, 1.04, 1.35]	OpticalFilter525 abc.BandOpticalFilter at 0x140603530113296\nFields:\n bandwidth_in_nm: 50.0\n center_wavelength_in_nm: 525.0\n description: The band-pass filter used to isolate the green emitted light after passing through a dichroic (Chroma, No. 532rdc) that reflected green and passed red fluorescence.\n filter_type: Bandpass\n manufacturer: Chroma\n model: 525/50m\n	OpticalFilter470 abc.BandOpticalFilter at 0x140603529377648\nFields:\n bandwidth_in_nm: 24.0\n center_wavelength_in_nm: 473.0\n description: The band-pass filter used to isolate the 470 nm excitation light.\n filter_type: Bandpass\n manufacturer: Chroma\n model: ET473/24\n
3	Caudoputamen	dLight1.3b abc.Indicator at 0x140603529377984\nFields:\n description: green dopamine sensor\n label: pAAV-CAG-dLight1.3b(AAV5)\n	FiberArray abc.OpticalFiber at 0x140603529377312\nFields:\n core_diameter_in_um: 34.0\n description: The optical fiber used in a multi-fiber arrays configuration, fabricated in-house to enable large scale measurements across deep brain volumes. Bare fibers were cut into pieces (ca. 3cm) then mounted under a microscope into 55-60μm diameter holes in a custom 3D printed grid (3mm W x 5mm L, Boston Micro Fabrication), measured under a dissection microscope to target a particular depth beneath the grid, and secured in place with UV glue (Norland Optical Adhesive 61). Each array contained between 30 and 103 fibers separated by a minimum of 220μm radially and 250μm axially. Separation was calculated to achieve maximal coverage of the striatum volume with no overlap in the collection fields of individual fibers. Fibers were cut with a fiber scribe, and the distal ends were inspected to ensure a uniform cut, and re-cut as necessary. Distal ends were then glued inside an 1cm ca. section of polyimide tube (0.8-1.3mm diameter, MicroLumen) then cut with a fresh razorblade. The bundled fibers inside the tube were then polished on fine grained polishing paper (ThorLabs,polished first with 6 μm, followed by 3 μm) to create a smooth, uniform fiber bundle surface for imaging. A larger diameter post was mounted on one side of the plastic grid to facilitate holding during implantation.\n manufacturer: Fiber Optics Tech\n model: not specified\n numerical_aperture: 0.66\n	ExcitationSource470 abc.ExcitationSource at 0x140603529376976\nFields:\n description: Blue excitation light (470 nm LED, Thorlabs, No. SOLIS-470C) and violet excitation light (for the isosbestic control)\nwere coupled into the optic fiber such that a power of 0.75 mW was delivered to the fiber tip.\nThen, 470 nm and 405 nm excitation were alternated at 100 Hz using a waveform generator,\neach filtered with a corresponding filter.\n\n excitation_wavelength_in_nm: 470.0\n illumination_type: LED\n manufacturer: Thorlabs\n model: SOLIS-470C\n	CMOSCamera pynwb.device.Device at 0x140603530107856\nFields:\n description: A tube lens in each path (Thor labs, No TTL165-A) focused emission light onto the CMOS sensors of the cameras to form an image of the fiber bundle.\n manufacturer: Hamamatsu Photonics, Orca Fusion BT Gen III\n	DichroicMirror1 abc.DichroicMirror at 0x140603529376640\nFields:\n cut_on_wavelength_in_nm: 532.0\n description: The dichroic mirror used to reflect green and pass red fluorescence\n manufacturer: Chroma Tech Corp\n model: ZT532rdc\n reflection_band_in_nm: [405. 532.]\n transmission_band_in_nm: [545. 750.]\n	[495, 685, 260]	False	[0.3, 1.15, 2.05]	OpticalFilter525 abc.BandOpticalFilter at 0x140603530113296\nFields:\n bandwidth_in_nm: 50.0\n center_wavelength_in_nm: 525.0\n description: The band-pass filter used to isolate the green emitted light after passing through a dichroic (Chroma, No. 532rdc) that reflected green and passed red fluorescence.\n filter_type: Bandpass\n manufacturer: Chroma\n model: 525/50m\n	OpticalFilter470 abc.BandOpticalFilter at 0x140603529377648\nFields:\n bandwidth_in_nm: 24.0\n center_wavelength_in_nm: 473.0\n description: The band-pass filter used to isolate the 470 nm excitation light.\n filter_type: Bandpass\n manufacturer: Chroma\n model: ET473/24\n