# install cell skipped during CI (deps preinstalled into system Python)

from stream_nwbfile import stream_nwbfile
import numpy as np
import matplotlib.pyplot as plt

DANDISET_ID = '000971'
file_path = 'sub-112-283/sub-112-283_ses-FP-PS-2019-06-20T09-32-04_behavior.nwb'
nwbfile, io = stream_nwbfile(DANDISET_ID, file_path)
display(nwbfile)

/opt/hostedtoolcache/Python/3.13.15/x64/lib/python3.13/site-packages/hdmf/spec/namespace.py:484: UserWarning: Schema conflict(s) detected in namespace 'ndx-fiber-photometry': 
 ndx-fiber-photometry defines OpticalFiber.model as an attribute (dtype: text) while the core schema defines it as a link to DeviceModel.
ndx-fiber-photometry defines ExcitationSource.model as an attribute (dtype: text) while the core schema defines it as a link to DeviceModel.
ndx-fiber-photometry defines Photodetector.model as an attribute (dtype: text) while the core schema defines it as a link to DeviceModel.
ndx-fiber-photometry defines DichroicMirror.model as an attribute (dtype: text) while the core schema defines it as a link to DeviceModel.
ndx-fiber-photometry defines BandOpticalFilter.model as an attribute (dtype: text) while the core schema defines it as a link to DeviceModel.
ndx-fiber-photometry defines EdgeOpticalFilter.model as an attribute (dtype: text) while the core schema defines it as a link to DeviceModel. 
This may cause compatibility issues. Please update the extension version if possible or install an older version of the core schema that is compatible.
  self._check_namespace_conflicts(extension_ns_name=ns_name,

/opt/hostedtoolcache/Python/3.13.15/x64/lib/python3.13/site-packages/hdmf/build/objectmapper.py:1399: UserWarning: Device.model was detected as a string, but NWB 2.9 specifies Device.model as a link to a DeviceModel. Remapping "4 ports Fluorescence Mini Cube - GCaMP" to a new DeviceModel.
  override = self.__get_override_carg(argname, builder, manager)
/opt/hostedtoolcache/Python/3.13.15/x64/lib/python3.13/site-packages/hdmf/build/objectmapper.py:1399: UserWarning: Device.model was detected as a string, but NWB 2.9 specifies Device.model as a link to a DeviceModel. Remapping "Connectorized LED" to a new DeviceModel.
  override = self.__get_override_carg(argname, builder, manager)
/opt/hostedtoolcache/Python/3.13.15/x64/lib/python3.13/site-packages/hdmf/build/objectmapper.py:1399: UserWarning: Device.model was detected as a string, but NWB 2.9 specifies Device.model as a link to a DeviceModel. Remapping "Fiber Optic Implant" to a new DeviceModel.
  override = self.__get_override_carg(argname, builder, manager)
/opt/hostedtoolcache/Python/3.13.15/x64/lib/python3.13/site-packages/hdmf/build/objectmapper.py:1399: UserWarning: Device.model was detected as a string, but NWB 2.9 specifies Device.model as a link to a DeviceModel. Remapping "Newport Visible Femtowatt Photoreceiver Module" to a new DeviceModel.
  override = self.__get_override_carg(argname, builder, manager)

# Photometry
fiber_photometry_responses = nwbfile.acquisition['fiber_photometry_response_series'].data[:]
dms_calcium_signal = fiber_photometry_responses[:, 0]
dms_isosbestic_control = fiber_photometry_responses[:, 1]
dls_calcium_signal = fiber_photometry_responses[:, 2]
dls_isosbestic_control = fiber_photometry_responses[:, 3]
fs = nwbfile.acquisition['fiber_photometry_response_series'].rate
timestamps = np.arange(0, len(dms_calcium_signal) / fs, 1/fs)

# Behavior
left_nose_poke_times = nwbfile.processing['behavior'].data_interfaces['left_nose_poke_times'].timestamps[:]
left_reward_times = nwbfile.processing['behavior'].data_interfaces['left_reward_times'].timestamps[:]
reward_port_intervals = nwbfile.processing['behavior'].data_interfaces['behavioral_epochs'].interval_series['reward_port_intervals']
reward_port_interval_data = reward_port_intervals.data[:]
reward_port_interval_times = reward_port_intervals.timestamps[:]
reward_port_entry_times = reward_port_interval_times[reward_port_interval_data==1]
reward_port_exit_times = reward_port_interval_times[reward_port_interval_data==-1]

t_start = 2000
t_end = 2100
photometry_slice = slice(int(t_start * fs), int(t_end * fs))
left_nose_poke_mask = np.logical_and(left_nose_poke_times >= t_start, left_nose_poke_times < t_end)
left_reward_mask = np.logical_and(left_reward_times >= t_start, left_reward_times < t_end)
reward_port_interval_mask = np.logical_and(reward_port_entry_times >= t_start, reward_port_entry_times < t_end)
lineoffsets = 200
linelengths = 50
y = np.arange(170, 180, 0.1)
alpha = 0.3
ylim = [165, 235]

fix, ax = plt.subplots(2, 1, figsize=(10, 10), sharex=True)
ax[0].plot(timestamps[photometry_slice], dms_calcium_signal[photometry_slice], label='Calcium Signal')
ax[0].plot(timestamps[photometry_slice], dms_isosbestic_control[photometry_slice], label='Isosbestic Control')
ax[0].eventplot(left_nose_poke_times[left_nose_poke_mask], lineoffsets=lineoffsets, linelengths=linelengths, color='r', label='Left Nose Poke')
ax[0].eventplot(left_reward_times[left_reward_mask], lineoffsets=lineoffsets, linelengths=linelengths, color='g', label='Left Reward')
for i, (reward_port_entry_time, reward_port_exit_time) in enumerate(zip(reward_port_entry_times[reward_port_interval_mask], reward_port_exit_times[reward_port_interval_mask])):
    x1 = reward_port_entry_time*np.ones(len(y))
    x2 = reward_port_exit_time*np.ones(len(y))
    if i == 0:
        ax[0].fill_betweenx(y, x1, x2, color='b', alpha=alpha, label='In Reward Port')
    else:
        ax[0].fill_betweenx(y, x1, x2, color='b', alpha=alpha)
ax[0].set_ylim(ylim)
ax[0].set_title('DMS')
ax[0].legend()
ax[0].set_ylabel('Fluorescence (a.u.)')

ax[1].plot(timestamps[photometry_slice], dls_calcium_signal[photometry_slice], label='Calcium Signal')
ax[1].plot(timestamps[photometry_slice], dls_isosbestic_control[photometry_slice], label='Isosbestic Control')
ax[1].eventplot(left_nose_poke_times[left_nose_poke_mask], lineoffsets=lineoffsets, linelengths=linelengths, color='r', label='Left Nose Poke')
ax[1].eventplot(left_reward_times[left_reward_mask], lineoffsets=lineoffsets, linelengths=linelengths, color='g', label='Left Reward')
for i, (reward_port_entry_time, reward_port_exit_time) in enumerate(zip(reward_port_entry_times[reward_port_interval_mask], reward_port_exit_times[reward_port_interval_mask])):
    x1 = reward_port_entry_time*np.ones(len(y))
    x2 = reward_port_exit_time*np.ones(len(y))
    if i == 0:
        ax[1].fill_betweenx(y, x1, x2, color='b', alpha=alpha, label='In Reward Port')
    else:
        ax[1].fill_betweenx(y, x1, x2, color='b', alpha=alpha)
ax[1].set_ylim(ylim)
ax[1].set_title('DLS')
ax[1].legend()
ax[1].set_xlabel('Time (s)')
_ = ax[1].set_ylabel('Fluorescence (a.u.)')

t_start = 2000
t_end = 2020
photometry_slice = slice(int(t_start * fs), int(t_end * fs))
left_nose_poke_mask = np.logical_and(left_nose_poke_times >= t_start, left_nose_poke_times < t_end)
left_reward_mask = np.logical_and(left_reward_times >= t_start, left_reward_times < t_end)
reward_port_interval_mask = np.logical_and(reward_port_entry_times >= t_start, reward_port_entry_times < t_end)
lineoffsets = 200
linelengths = 50
y = np.arange(170, 180, 0.1)
alpha = 0.3
ylim = [165, 235]

fix, ax = plt.subplots(2, 1, figsize=(10, 10), sharex=True)
ax[0].plot(timestamps[photometry_slice], dms_calcium_signal[photometry_slice], label='Calcium Signal')
ax[0].plot(timestamps[photometry_slice], dms_isosbestic_control[photometry_slice], label='Isosbestic Control')
ax[0].eventplot(left_nose_poke_times[left_nose_poke_mask], lineoffsets=lineoffsets, linelengths=linelengths, color='r', label='Left Nose Poke')
ax[0].eventplot(left_reward_times[left_reward_mask], lineoffsets=lineoffsets, linelengths=linelengths, color='g', label='Left Reward')
for i, (reward_port_entry_time, reward_port_exit_time) in enumerate(zip(reward_port_entry_times[reward_port_interval_mask], reward_port_exit_times[reward_port_interval_mask])):
    x1 = reward_port_entry_time*np.ones(len(y))
    x2 = reward_port_exit_time*np.ones(len(y))
    if i == 0:
        ax[0].fill_betweenx(y, x1, x2, color='b', alpha=alpha, label='In Reward Port')
    else:
        ax[0].fill_betweenx(y, x1, x2, color='b', alpha=alpha)
ax[0].set_ylim(ylim)
ax[0].set_title('DMS')
ax[0].legend()
ax[0].set_ylabel('Fluorescence (a.u.)')

ax[1].plot(timestamps[photometry_slice], dls_calcium_signal[photometry_slice], label='Calcium Signal')
ax[1].plot(timestamps[photometry_slice], dls_isosbestic_control[photometry_slice], label='Isosbestic Control')
ax[1].eventplot(left_nose_poke_times[left_nose_poke_mask], lineoffsets=lineoffsets, linelengths=linelengths, color='r', label='Left Nose Poke')
ax[1].eventplot(left_reward_times[left_reward_mask], lineoffsets=lineoffsets, linelengths=linelengths, color='g', label='Left Reward')
for i, (reward_port_entry_time, reward_port_exit_time) in enumerate(zip(reward_port_entry_times[reward_port_interval_mask], reward_port_exit_times[reward_port_interval_mask])):
    x1 = reward_port_entry_time*np.ones(len(y))
    x2 = reward_port_exit_time*np.ones(len(y))
    if i == 0:
        ax[1].fill_betweenx(y, x1, x2, color='b', alpha=alpha, label='In Reward Port')
    else:
        ax[1].fill_betweenx(y, x1, x2, color='b', alpha=alpha)
ax[1].set_ylim(ylim)
ax[1].set_title('DLS')
ax[1].legend()
ax[1].set_xlabel('Time (s)')
_ = ax[1].set_ylabel('Fluorescence (a.u.)')

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Installing requirements¶

Fiber Photometry Example Session¶

root (NWBFile)

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	location	indicator	optical_fiber	excitation_source	photodetector	dichroic_mirror	coordinates	commanded_voltage_series	emission_filter	excitation_filter
id
0	DMS	dms_green_fluorophore abc.Indicator at 0x139967255131024\nFields:\n description: Mice for fiber photometry experiments received infusions of 1ml of AAV5-CAG-FLEX-jGCaMP7b-WPRE (1.02e13 vg/mL, Addgene, lot 18-429) into lateral SNc (AP 3.1, ML 1.3, DV 4.2) in one hemisphere and medial SNc (AP 3.1, ML 0.8, DV 4.7) in the other. Hemispheres were counterbalanced between mice.\n injection_coordinates_in_mm: [3.1 0.8 4.7]\n injection_location: medial SNc\n label: GCaMP7b\n manufacturer: Addgene\n	optical_fiber abc.OpticalFiber at 0x139967234860976\nFields:\n core_diameter_in_um: 400.0\n description: Fiber optic implants (Doric Lenses; 400 um, 0.48 NA) were placed above DMS (AP 0.8, ML 1.5, DV 2.8) and DLS (AP 0.1, ML 2.8, DV 3.5). The DMS implant was placed in the hemisphere receiving a medial SNc viral injection, while the DLS implant was placed in the hemisphere receiving a lateral SNc viral injection. Calcium signals from dopamine terminals in DMS and DLS were recorded during RI30, on the first and last days of RI60/RR20 training as well as on both footshock probes for each mouse. All recordings were done using a fiber photometry rig with optical components from Doric lenses controlled by a real-time processor from Tucker Davis Technologies (TDT; RZ5P). TDT Synapse software was used for data acquisition.\n manufacturer: Doric Lenses\n model: Fiber Optic Implant pynwb.device.DeviceModel at 0x139967254695184\nFields:\n description: Fiber optic implants (Doric Lenses; 400 um, 0.48 NA) were placed above DMS (AP 0.8, ML 1.5, DV 2.8) and DLS (AP 0.1, ML 2.8, DV 3.5). The DMS implant was placed in the hemisphere receiving a medial SNc viral injection, while the DLS implant was placed in the hemisphere receiving a lateral SNc viral injection. Calcium signals from dopamine terminals in DMS and DLS were recorded during RI30, on the first and last days of RI60/RR20 training as well as on both footshock probes for each mouse. All recordings were done using a fiber photometry rig with optical components from Doric lenses controlled by a real-time processor from Tucker Davis Technologies (TDT; RZ5P). TDT Synapse software was used for data acquisition.\n manufacturer: Doric Lenses\n\n numerical_aperture: 0.48\n	excitation_source_calcium_signal abc.ExcitationSource at 0x139967234860640\nFields:\n description: 465nm and 405nm LEDs were modulated at 211 Hz and 330 Hz, respectively, for DMS probes. 465nm and 405nm LEDs were modulated at 450 Hz and 270 Hz, respectively for DLS probes. LED currents were adjusted in order to return a voltage between 150-200mV for each signal, were offset by 5 mA, were demodulated using a 4 Hz lowpass frequency filter.\n excitation_wavelength_in_nm: 465.0\n illumination_type: LED\n manufacturer: Doric Lenses\n model: Connectorized LED pynwb.device.DeviceModel at 0x139967254681312\nFields:\n description: 465nm and 405nm LEDs were modulated at 211 Hz and 330 Hz, respectively, for DMS probes. 465nm and 405nm LEDs were modulated at 450 Hz and 270 Hz, respectively for DLS probes. LED currents were adjusted in order to return a voltage between 150-200mV for each signal, were offset by 5 mA, were demodulated using a 4 Hz lowpass frequency filter.\n manufacturer: Doric Lenses\n\n	photodetector abc.Photodetector at 0x139967234861312\nFields:\n description: This battery-operated photoreceiver has high gain and detects CW light signals in the sub-picowatt to nanowatt range. When used in conjunction with a modulated light source and a lock-in amplifier to reduce the measurement bandwidth, it achieves sensitivity levels in the femtowatt range. Doric offer this Newport product with add-on fiber optic adapter that improves coupling efficiency between the large core, high NA optical fibers used in Fiber Photometry and relatively small detector area. Its output analog voltage (0-5 V) can be monitored with an oscilloscope or with a DAQ board to record the data with a computer.\n detected_wavelength_in_nm: 525.0\n detector_type: photodiode\n gain: 10000000000.0\n manufacturer: Doric Lenses\n model: Newport Visible Femtowatt Photoreceiver Module pynwb.device.DeviceModel at 0x139967254696272\nFields:\n description: This battery-operated photoreceiver has high gain and detects CW light signals in the sub-picowatt to nanowatt range. When used in conjunction with a modulated light source and a lock-in amplifier to reduce the measurement bandwidth, it achieves sensitivity levels in the femtowatt range. Doric offer this Newport product with add-on fiber optic adapter that improves coupling efficiency between the large core, high NA optical fibers used in Fiber Photometry and relatively small detector area. Its output analog voltage (0-5 V) can be monitored with an oscilloscope or with a DAQ board to record the data with a computer.\n manufacturer: Doric Lenses\n\n	dichroic_mirror abc.DichroicMirror at 0x139967234859632\nFields:\n description: Dual excitation band fiber photometry measurements use a Fluorescence Mini Cube with 4 ports: one port for the functional fluorescence excitation light, one for the isosbestic excitation, one for the fluorescence detection, and one for the sample. The cube has dichroic mirrors to combine isosbestic and fluorescence excitations and separate the fluorescence emission and narrow bandpass filters limiting the excitation fluorescence spectrum.\n manufacturer: Doric Lenses\n model: 4 ports Fluorescence Mini Cube - GCaMP pynwb.device.DeviceModel at 0x139967234859296\nFields:\n description: Dual excitation band fiber photometry measurements use a Fluorescence Mini Cube with 4 ports: one port for the functional fluorescence excitation light, one for the isosbestic excitation, one for the fluorescence detection, and one for the sample. The cube has dichroic mirrors to combine isosbestic and fluorescence excitations and separate the fluorescence emission and narrow bandpass filters limiting the excitation fluorescence spectrum.\n manufacturer: Doric Lenses\n\n	[0.8, 1.5, 2.8]	commanded_voltage_series_dms_calcium_signal abc.CommandedVoltageSeries at 0x139967255127184\nFields:\n comments: no comments\n conversion: 1.0\n data: <HDF5 dataset "data": shape (22570496,), type "<f4">\n description: The commanded voltage for the DMS calcium signal.\n frequency: 211.0\n frequency__unit: hertz\n offset: 0.0\n rate: 6103.515625\n resolution: -1.0\n starting_time: 0.0\n starting_time_unit: seconds\n unit: volts\n	emission_filter abc.BandOpticalFilter at 0x139967234860304\nFields:\n bandwidth_in_nm: 50.0\n center_wavelength_in_nm: 525.0\n description: Dual excitation band fiber photometry measurements use a Fluorescence Mini Cube with 4 ports: one port for the functional fluorescence excitation light, one for the isosbestic excitation, one for the fluorescence detection, and one for the sample. The cube has dichroic mirrors to combine isosbestic and fluorescence excitations and separate the fluorescence emission and narrow bandpass filters limiting the excitation fluorescence spectrum.\n filter_type: Bandpass\n manufacturer: Doric Lenses\n model: 4 ports Fluorescence Mini Cube - GCaMP pynwb.device.DeviceModel at 0x139967255131664\nFields:\n description: Dual excitation band fiber photometry measurements use a Fluorescence Mini Cube with 4 ports: one port for the functional fluorescence excitation light, one for the isosbestic excitation, one for the fluorescence detection, and one for the sample. The cube has dichroic mirrors to combine isosbestic and fluorescence excitations and separate the fluorescence emission and narrow bandpass filters limiting the excitation fluorescence spectrum.\n manufacturer: Doric Lenses\n\n	excitation_filter abc.BandOpticalFilter at 0x139967255132304\nFields:\n bandwidth_in_nm: 30.0\n center_wavelength_in_nm: 475.0\n description: Dual excitation band fiber photometry measurements use a Fluorescence Mini Cube with 4 ports: one port for the functional fluorescence excitation light, one for the isosbestic excitation, one for the fluorescence detection, and one for the sample. The cube has dichroic mirrors to combine isosbestic and fluorescence excitations and separate the fluorescence emission and narrow bandpass filters limiting the excitation fluorescence spectrum.\n filter_type: Bandpass\n manufacturer: Doric Lenses\n model: 4 ports Fluorescence Mini Cube - GCaMP pynwb.device.DeviceModel at 0x139967255131984\nFields:\n description: Dual excitation band fiber photometry measurements use a Fluorescence Mini Cube with 4 ports: one port for the functional fluorescence excitation light, one for the isosbestic excitation, one for the fluorescence detection, and one for the sample. The cube has dichroic mirrors to combine isosbestic and fluorescence excitations and separate the fluorescence emission and narrow bandpass filters limiting the excitation fluorescence spectrum.\n manufacturer: Doric Lenses\n\n
1	DMS	dms_green_fluorophore abc.Indicator at 0x139967255131024\nFields:\n description: Mice for fiber photometry experiments received infusions of 1ml of AAV5-CAG-FLEX-jGCaMP7b-WPRE (1.02e13 vg/mL, Addgene, lot 18-429) into lateral SNc (AP 3.1, ML 1.3, DV 4.2) in one hemisphere and medial SNc (AP 3.1, ML 0.8, DV 4.7) in the other. Hemispheres were counterbalanced between mice.\n injection_coordinates_in_mm: [3.1 0.8 4.7]\n injection_location: medial SNc\n label: GCaMP7b\n manufacturer: Addgene\n	optical_fiber abc.OpticalFiber at 0x139967234860976\nFields:\n core_diameter_in_um: 400.0\n description: Fiber optic implants (Doric Lenses; 400 um, 0.48 NA) were placed above DMS (AP 0.8, ML 1.5, DV 2.8) and DLS (AP 0.1, ML 2.8, DV 3.5). The DMS implant was placed in the hemisphere receiving a medial SNc viral injection, while the DLS implant was placed in the hemisphere receiving a lateral SNc viral injection. Calcium signals from dopamine terminals in DMS and DLS were recorded during RI30, on the first and last days of RI60/RR20 training as well as on both footshock probes for each mouse. All recordings were done using a fiber photometry rig with optical components from Doric lenses controlled by a real-time processor from Tucker Davis Technologies (TDT; RZ5P). TDT Synapse software was used for data acquisition.\n manufacturer: Doric Lenses\n model: Fiber Optic Implant pynwb.device.DeviceModel at 0x139967254695184\nFields:\n description: Fiber optic implants (Doric Lenses; 400 um, 0.48 NA) were placed above DMS (AP 0.8, ML 1.5, DV 2.8) and DLS (AP 0.1, ML 2.8, DV 3.5). The DMS implant was placed in the hemisphere receiving a medial SNc viral injection, while the DLS implant was placed in the hemisphere receiving a lateral SNc viral injection. Calcium signals from dopamine terminals in DMS and DLS were recorded during RI30, on the first and last days of RI60/RR20 training as well as on both footshock probes for each mouse. All recordings were done using a fiber photometry rig with optical components from Doric lenses controlled by a real-time processor from Tucker Davis Technologies (TDT; RZ5P). TDT Synapse software was used for data acquisition.\n manufacturer: Doric Lenses\n\n numerical_aperture: 0.48\n	excitation_source_isosbestic_control abc.ExcitationSource at 0x139967255132624\nFields:\n description: 465nm and 405nm LEDs were modulated at 211 Hz and 330 Hz, respectively, for DMS probes. 465nm and 405nm LEDs were modulated at 450 Hz and 270 Hz, respectively for DLS probes. LED currents were adjusted in order to return a voltage between 150-200mV for each signal, were offset by 5 mA, were demodulated using a 4 Hz lowpass frequency filter.\n excitation_wavelength_in_nm: 405.0\n illumination_type: LED\n manufacturer: Doric Lenses\n model: Connectorized LED pynwb.device.DeviceModel at 0x139967254681616\nFields:\n description: 465nm and 405nm LEDs were modulated at 211 Hz and 330 Hz, respectively, for DMS probes. 465nm and 405nm LEDs were modulated at 450 Hz and 270 Hz, respectively for DLS probes. LED currents were adjusted in order to return a voltage between 150-200mV for each signal, were offset by 5 mA, were demodulated using a 4 Hz lowpass frequency filter.\n manufacturer: Doric Lenses\n\n	photodetector abc.Photodetector at 0x139967234861312\nFields:\n description: This battery-operated photoreceiver has high gain and detects CW light signals in the sub-picowatt to nanowatt range. When used in conjunction with a modulated light source and a lock-in amplifier to reduce the measurement bandwidth, it achieves sensitivity levels in the femtowatt range. Doric offer this Newport product with add-on fiber optic adapter that improves coupling efficiency between the large core, high NA optical fibers used in Fiber Photometry and relatively small detector area. Its output analog voltage (0-5 V) can be monitored with an oscilloscope or with a DAQ board to record the data with a computer.\n detected_wavelength_in_nm: 525.0\n detector_type: photodiode\n gain: 10000000000.0\n manufacturer: Doric Lenses\n model: Newport Visible Femtowatt Photoreceiver Module pynwb.device.DeviceModel at 0x139967254696272\nFields:\n description: This battery-operated photoreceiver has high gain and detects CW light signals in the sub-picowatt to nanowatt range. When used in conjunction with a modulated light source and a lock-in amplifier to reduce the measurement bandwidth, it achieves sensitivity levels in the femtowatt range. Doric offer this Newport product with add-on fiber optic adapter that improves coupling efficiency between the large core, high NA optical fibers used in Fiber Photometry and relatively small detector area. Its output analog voltage (0-5 V) can be monitored with an oscilloscope or with a DAQ board to record the data with a computer.\n manufacturer: Doric Lenses\n\n	dichroic_mirror abc.DichroicMirror at 0x139967234859632\nFields:\n description: Dual excitation band fiber photometry measurements use a Fluorescence Mini Cube with 4 ports: one port for the functional fluorescence excitation light, one for the isosbestic excitation, one for the fluorescence detection, and one for the sample. The cube has dichroic mirrors to combine isosbestic and fluorescence excitations and separate the fluorescence emission and narrow bandpass filters limiting the excitation fluorescence spectrum.\n manufacturer: Doric Lenses\n model: 4 ports Fluorescence Mini Cube - GCaMP pynwb.device.DeviceModel at 0x139967234859296\nFields:\n description: Dual excitation band fiber photometry measurements use a Fluorescence Mini Cube with 4 ports: one port for the functional fluorescence excitation light, one for the isosbestic excitation, one for the fluorescence detection, and one for the sample. The cube has dichroic mirrors to combine isosbestic and fluorescence excitations and separate the fluorescence emission and narrow bandpass filters limiting the excitation fluorescence spectrum.\n manufacturer: Doric Lenses\n\n	[0.8, 1.5, 2.8]	commanded_voltage_series_dms_isosbestic_control abc.CommandedVoltageSeries at 0x139967254676448\nFields:\n comments: no comments\n conversion: 1.0\n data: <HDF5 dataset "data": shape (22570496,), type "<f4">\n description: The commanded voltage for the DMS isosbestic control.\n frequency: 330.0\n frequency__unit: hertz\n offset: 0.0\n rate: 6103.515625\n resolution: -1.0\n starting_time: 0.0\n starting_time_unit: seconds\n unit: volts\n	emission_filter abc.BandOpticalFilter at 0x139967234860304\nFields:\n bandwidth_in_nm: 50.0\n center_wavelength_in_nm: 525.0\n description: Dual excitation band fiber photometry measurements use a Fluorescence Mini Cube with 4 ports: one port for the functional fluorescence excitation light, one for the isosbestic excitation, one for the fluorescence detection, and one for the sample. The cube has dichroic mirrors to combine isosbestic and fluorescence excitations and separate the fluorescence emission and narrow bandpass filters limiting the excitation fluorescence spectrum.\n filter_type: Bandpass\n manufacturer: Doric Lenses\n model: 4 ports Fluorescence Mini Cube - GCaMP pynwb.device.DeviceModel at 0x139967255131664\nFields:\n description: Dual excitation band fiber photometry measurements use a Fluorescence Mini Cube with 4 ports: one port for the functional fluorescence excitation light, one for the isosbestic excitation, one for the fluorescence detection, and one for the sample. The cube has dichroic mirrors to combine isosbestic and fluorescence excitations and separate the fluorescence emission and narrow bandpass filters limiting the excitation fluorescence spectrum.\n manufacturer: Doric Lenses\n\n	isosbestic_excitation_filter abc.BandOpticalFilter at 0x139967255132944\nFields:\n bandwidth_in_nm: 10.0\n center_wavelength_in_nm: 405.0\n description: Dual excitation band fiber photometry measurements use a Fluorescence Mini Cube with 4 ports: one port for the functional fluorescence excitation light, one for the isosbestic excitation, one for the fluorescence detection, and one for the sample. The cube has dichroic mirrors to combine isosbestic and fluorescence excitations and separate the fluorescence emission and narrow bandpass filters limiting the excitation fluorescence spectrum.\n filter_type: Bandpass\n manufacturer: Doric Lenses\n model: 4 ports Fluorescence Mini Cube - GCaMP pynwb.device.DeviceModel at 0x139967235670096\nFields:\n description: Dual excitation band fiber photometry measurements use a Fluorescence Mini Cube with 4 ports: one port for the functional fluorescence excitation light, one for the isosbestic excitation, one for the fluorescence detection, and one for the sample. The cube has dichroic mirrors to combine isosbestic and fluorescence excitations and separate the fluorescence emission and narrow bandpass filters limiting the excitation fluorescence spectrum.\n manufacturer: Doric Lenses\n\n
2	DLS	dls_green_fluorophore abc.Indicator at 0x139967234859968\nFields:\n description: Mice for fiber photometry experiments received infusions of 1ml of AAV5-CAG-FLEX-jGCaMP7b-WPRE (1.02e13 vg/mL, Addgene, lot 18-429) into lateral SNc (AP 3.1, ML 1.3, DV 4.2) in one hemisphere and medial SNc (AP 3.1, ML 0.8, DV 4.7) in the other. Hemispheres were counterbalanced between mice.\n injection_coordinates_in_mm: [3.1 1.3 4.2]\n injection_location: lateral SNc\n label: GCaMP7b\n manufacturer: Addgene\n	optical_fiber abc.OpticalFiber at 0x139967234860976\nFields:\n core_diameter_in_um: 400.0\n description: Fiber optic implants (Doric Lenses; 400 um, 0.48 NA) were placed above DMS (AP 0.8, ML 1.5, DV 2.8) and DLS (AP 0.1, ML 2.8, DV 3.5). The DMS implant was placed in the hemisphere receiving a medial SNc viral injection, while the DLS implant was placed in the hemisphere receiving a lateral SNc viral injection. Calcium signals from dopamine terminals in DMS and DLS were recorded during RI30, on the first and last days of RI60/RR20 training as well as on both footshock probes for each mouse. All recordings were done using a fiber photometry rig with optical components from Doric lenses controlled by a real-time processor from Tucker Davis Technologies (TDT; RZ5P). TDT Synapse software was used for data acquisition.\n manufacturer: Doric Lenses\n model: Fiber Optic Implant pynwb.device.DeviceModel at 0x139967254695184\nFields:\n description: Fiber optic implants (Doric Lenses; 400 um, 0.48 NA) were placed above DMS (AP 0.8, ML 1.5, DV 2.8) and DLS (AP 0.1, ML 2.8, DV 3.5). The DMS implant was placed in the hemisphere receiving a medial SNc viral injection, while the DLS implant was placed in the hemisphere receiving a lateral SNc viral injection. Calcium signals from dopamine terminals in DMS and DLS were recorded during RI30, on the first and last days of RI60/RR20 training as well as on both footshock probes for each mouse. All recordings were done using a fiber photometry rig with optical components from Doric lenses controlled by a real-time processor from Tucker Davis Technologies (TDT; RZ5P). TDT Synapse software was used for data acquisition.\n manufacturer: Doric Lenses\n\n numerical_aperture: 0.48\n	excitation_source_calcium_signal abc.ExcitationSource at 0x139967234860640\nFields:\n description: 465nm and 405nm LEDs were modulated at 211 Hz and 330 Hz, respectively, for DMS probes. 465nm and 405nm LEDs were modulated at 450 Hz and 270 Hz, respectively for DLS probes. LED currents were adjusted in order to return a voltage between 150-200mV for each signal, were offset by 5 mA, were demodulated using a 4 Hz lowpass frequency filter.\n excitation_wavelength_in_nm: 465.0\n illumination_type: LED\n manufacturer: Doric Lenses\n model: Connectorized LED pynwb.device.DeviceModel at 0x139967254681312\nFields:\n description: 465nm and 405nm LEDs were modulated at 211 Hz and 330 Hz, respectively, for DMS probes. 465nm and 405nm LEDs were modulated at 450 Hz and 270 Hz, respectively for DLS probes. LED currents were adjusted in order to return a voltage between 150-200mV for each signal, were offset by 5 mA, were demodulated using a 4 Hz lowpass frequency filter.\n manufacturer: Doric Lenses\n\n	photodetector abc.Photodetector at 0x139967234861312\nFields:\n description: This battery-operated photoreceiver has high gain and detects CW light signals in the sub-picowatt to nanowatt range. When used in conjunction with a modulated light source and a lock-in amplifier to reduce the measurement bandwidth, it achieves sensitivity levels in the femtowatt range. Doric offer this Newport product with add-on fiber optic adapter that improves coupling efficiency between the large core, high NA optical fibers used in Fiber Photometry and relatively small detector area. Its output analog voltage (0-5 V) can be monitored with an oscilloscope or with a DAQ board to record the data with a computer.\n detected_wavelength_in_nm: 525.0\n detector_type: photodiode\n gain: 10000000000.0\n manufacturer: Doric Lenses\n model: Newport Visible Femtowatt Photoreceiver Module pynwb.device.DeviceModel at 0x139967254696272\nFields:\n description: This battery-operated photoreceiver has high gain and detects CW light signals in the sub-picowatt to nanowatt range. When used in conjunction with a modulated light source and a lock-in amplifier to reduce the measurement bandwidth, it achieves sensitivity levels in the femtowatt range. Doric offer this Newport product with add-on fiber optic adapter that improves coupling efficiency between the large core, high NA optical fibers used in Fiber Photometry and relatively small detector area. Its output analog voltage (0-5 V) can be monitored with an oscilloscope or with a DAQ board to record the data with a computer.\n manufacturer: Doric Lenses\n\n	dichroic_mirror abc.DichroicMirror at 0x139967234859632\nFields:\n description: Dual excitation band fiber photometry measurements use a Fluorescence Mini Cube with 4 ports: one port for the functional fluorescence excitation light, one for the isosbestic excitation, one for the fluorescence detection, and one for the sample. The cube has dichroic mirrors to combine isosbestic and fluorescence excitations and separate the fluorescence emission and narrow bandpass filters limiting the excitation fluorescence spectrum.\n manufacturer: Doric Lenses\n model: 4 ports Fluorescence Mini Cube - GCaMP pynwb.device.DeviceModel at 0x139967234859296\nFields:\n description: Dual excitation band fiber photometry measurements use a Fluorescence Mini Cube with 4 ports: one port for the functional fluorescence excitation light, one for the isosbestic excitation, one for the fluorescence detection, and one for the sample. The cube has dichroic mirrors to combine isosbestic and fluorescence excitations and separate the fluorescence emission and narrow bandpass filters limiting the excitation fluorescence spectrum.\n manufacturer: Doric Lenses\n\n	[0.1, 2.8, 3.5]	commanded_voltage_series_dls_calcium_signal abc.CommandedVoltageSeries at 0x139967234853584\nFields:\n comments: no comments\n conversion: 1.0\n data: <HDF5 dataset "data": shape (22570496,), type "<f4">\n description: The commanded voltage for the DLS calcium signal.\n frequency: 450.0\n frequency__unit: hertz\n offset: 0.0\n rate: 6103.515625\n resolution: -1.0\n starting_time: 0.0\n starting_time_unit: seconds\n unit: volts\n	emission_filter abc.BandOpticalFilter at 0x139967234860304\nFields:\n bandwidth_in_nm: 50.0\n center_wavelength_in_nm: 525.0\n description: Dual excitation band fiber photometry measurements use a Fluorescence Mini Cube with 4 ports: one port for the functional fluorescence excitation light, one for the isosbestic excitation, one for the fluorescence detection, and one for the sample. The cube has dichroic mirrors to combine isosbestic and fluorescence excitations and separate the fluorescence emission and narrow bandpass filters limiting the excitation fluorescence spectrum.\n filter_type: Bandpass\n manufacturer: Doric Lenses\n model: 4 ports Fluorescence Mini Cube - GCaMP pynwb.device.DeviceModel at 0x139967255131664\nFields:\n description: Dual excitation band fiber photometry measurements use a Fluorescence Mini Cube with 4 ports: one port for the functional fluorescence excitation light, one for the isosbestic excitation, one for the fluorescence detection, and one for the sample. The cube has dichroic mirrors to combine isosbestic and fluorescence excitations and separate the fluorescence emission and narrow bandpass filters limiting the excitation fluorescence spectrum.\n manufacturer: Doric Lenses\n\n	excitation_filter abc.BandOpticalFilter at 0x139967255132304\nFields:\n bandwidth_in_nm: 30.0\n center_wavelength_in_nm: 475.0\n description: Dual excitation band fiber photometry measurements use a Fluorescence Mini Cube with 4 ports: one port for the functional fluorescence excitation light, one for the isosbestic excitation, one for the fluorescence detection, and one for the sample. The cube has dichroic mirrors to combine isosbestic and fluorescence excitations and separate the fluorescence emission and narrow bandpass filters limiting the excitation fluorescence spectrum.\n filter_type: Bandpass\n manufacturer: Doric Lenses\n model: 4 ports Fluorescence Mini Cube - GCaMP pynwb.device.DeviceModel at 0x139967255131984\nFields:\n description: Dual excitation band fiber photometry measurements use a Fluorescence Mini Cube with 4 ports: one port for the functional fluorescence excitation light, one for the isosbestic excitation, one for the fluorescence detection, and one for the sample. The cube has dichroic mirrors to combine isosbestic and fluorescence excitations and separate the fluorescence emission and narrow bandpass filters limiting the excitation fluorescence spectrum.\n manufacturer: Doric Lenses\n\n
3	DLS	dls_green_fluorophore abc.Indicator at 0x139967234859968\nFields:\n description: Mice for fiber photometry experiments received infusions of 1ml of AAV5-CAG-FLEX-jGCaMP7b-WPRE (1.02e13 vg/mL, Addgene, lot 18-429) into lateral SNc (AP 3.1, ML 1.3, DV 4.2) in one hemisphere and medial SNc (AP 3.1, ML 0.8, DV 4.7) in the other. Hemispheres were counterbalanced between mice.\n injection_coordinates_in_mm: [3.1 1.3 4.2]\n injection_location: lateral SNc\n label: GCaMP7b\n manufacturer: Addgene\n	optical_fiber abc.OpticalFiber at 0x139967234860976\nFields:\n core_diameter_in_um: 400.0\n description: Fiber optic implants (Doric Lenses; 400 um, 0.48 NA) were placed above DMS (AP 0.8, ML 1.5, DV 2.8) and DLS (AP 0.1, ML 2.8, DV 3.5). The DMS implant was placed in the hemisphere receiving a medial SNc viral injection, while the DLS implant was placed in the hemisphere receiving a lateral SNc viral injection. Calcium signals from dopamine terminals in DMS and DLS were recorded during RI30, on the first and last days of RI60/RR20 training as well as on both footshock probes for each mouse. All recordings were done using a fiber photometry rig with optical components from Doric lenses controlled by a real-time processor from Tucker Davis Technologies (TDT; RZ5P). TDT Synapse software was used for data acquisition.\n manufacturer: Doric Lenses\n model: Fiber Optic Implant pynwb.device.DeviceModel at 0x139967254695184\nFields:\n description: Fiber optic implants (Doric Lenses; 400 um, 0.48 NA) were placed above DMS (AP 0.8, ML 1.5, DV 2.8) and DLS (AP 0.1, ML 2.8, DV 3.5). The DMS implant was placed in the hemisphere receiving a medial SNc viral injection, while the DLS implant was placed in the hemisphere receiving a lateral SNc viral injection. Calcium signals from dopamine terminals in DMS and DLS were recorded during RI30, on the first and last days of RI60/RR20 training as well as on both footshock probes for each mouse. All recordings were done using a fiber photometry rig with optical components from Doric lenses controlled by a real-time processor from Tucker Davis Technologies (TDT; RZ5P). TDT Synapse software was used for data acquisition.\n manufacturer: Doric Lenses\n\n numerical_aperture: 0.48\n	excitation_source_isosbestic_control abc.ExcitationSource at 0x139967255132624\nFields:\n description: 465nm and 405nm LEDs were modulated at 211 Hz and 330 Hz, respectively, for DMS probes. 465nm and 405nm LEDs were modulated at 450 Hz and 270 Hz, respectively for DLS probes. LED currents were adjusted in order to return a voltage between 150-200mV for each signal, were offset by 5 mA, were demodulated using a 4 Hz lowpass frequency filter.\n excitation_wavelength_in_nm: 405.0\n illumination_type: LED\n manufacturer: Doric Lenses\n model: Connectorized LED pynwb.device.DeviceModel at 0x139967254681616\nFields:\n description: 465nm and 405nm LEDs were modulated at 211 Hz and 330 Hz, respectively, for DMS probes. 465nm and 405nm LEDs were modulated at 450 Hz and 270 Hz, respectively for DLS probes. LED currents were adjusted in order to return a voltage between 150-200mV for each signal, were offset by 5 mA, were demodulated using a 4 Hz lowpass frequency filter.\n manufacturer: Doric Lenses\n\n	photodetector abc.Photodetector at 0x139967234861312\nFields:\n description: This battery-operated photoreceiver has high gain and detects CW light signals in the sub-picowatt to nanowatt range. When used in conjunction with a modulated light source and a lock-in amplifier to reduce the measurement bandwidth, it achieves sensitivity levels in the femtowatt range. Doric offer this Newport product with add-on fiber optic adapter that improves coupling efficiency between the large core, high NA optical fibers used in Fiber Photometry and relatively small detector area. Its output analog voltage (0-5 V) can be monitored with an oscilloscope or with a DAQ board to record the data with a computer.\n detected_wavelength_in_nm: 525.0\n detector_type: photodiode\n gain: 10000000000.0\n manufacturer: Doric Lenses\n model: Newport Visible Femtowatt Photoreceiver Module pynwb.device.DeviceModel at 0x139967254696272\nFields:\n description: This battery-operated photoreceiver has high gain and detects CW light signals in the sub-picowatt to nanowatt range. When used in conjunction with a modulated light source and a lock-in amplifier to reduce the measurement bandwidth, it achieves sensitivity levels in the femtowatt range. Doric offer this Newport product with add-on fiber optic adapter that improves coupling efficiency between the large core, high NA optical fibers used in Fiber Photometry and relatively small detector area. Its output analog voltage (0-5 V) can be monitored with an oscilloscope or with a DAQ board to record the data with a computer.\n manufacturer: Doric Lenses\n\n	dichroic_mirror abc.DichroicMirror at 0x139967234859632\nFields:\n description: Dual excitation band fiber photometry measurements use a Fluorescence Mini Cube with 4 ports: one port for the functional fluorescence excitation light, one for the isosbestic excitation, one for the fluorescence detection, and one for the sample. The cube has dichroic mirrors to combine isosbestic and fluorescence excitations and separate the fluorescence emission and narrow bandpass filters limiting the excitation fluorescence spectrum.\n manufacturer: Doric Lenses\n model: 4 ports Fluorescence Mini Cube - GCaMP pynwb.device.DeviceModel at 0x139967234859296\nFields:\n description: Dual excitation band fiber photometry measurements use a Fluorescence Mini Cube with 4 ports: one port for the functional fluorescence excitation light, one for the isosbestic excitation, one for the fluorescence detection, and one for the sample. The cube has dichroic mirrors to combine isosbestic and fluorescence excitations and separate the fluorescence emission and narrow bandpass filters limiting the excitation fluorescence spectrum.\n manufacturer: Doric Lenses\n\n	[0.1, 2.8, 3.5]	commanded_voltage_series_dls_isosbestic_control abc.CommandedVoltageSeries at 0x139967255126544\nFields:\n comments: no comments\n conversion: 1.0\n data: <HDF5 dataset "data": shape (22570496,), type "<f4">\n description: The commanded voltage for the DLS isosbestic control.\n frequency: 270.0\n frequency__unit: hertz\n offset: 0.0\n rate: 6103.515625\n resolution: -1.0\n starting_time: 0.0\n starting_time_unit: seconds\n unit: volts\n	emission_filter abc.BandOpticalFilter at 0x139967234860304\nFields:\n bandwidth_in_nm: 50.0\n center_wavelength_in_nm: 525.0\n description: Dual excitation band fiber photometry measurements use a Fluorescence Mini Cube with 4 ports: one port for the functional fluorescence excitation light, one for the isosbestic excitation, one for the fluorescence detection, and one for the sample. The cube has dichroic mirrors to combine isosbestic and fluorescence excitations and separate the fluorescence emission and narrow bandpass filters limiting the excitation fluorescence spectrum.\n filter_type: Bandpass\n manufacturer: Doric Lenses\n model: 4 ports Fluorescence Mini Cube - GCaMP pynwb.device.DeviceModel at 0x139967255131664\nFields:\n description: Dual excitation band fiber photometry measurements use a Fluorescence Mini Cube with 4 ports: one port for the functional fluorescence excitation light, one for the isosbestic excitation, one for the fluorescence detection, and one for the sample. The cube has dichroic mirrors to combine isosbestic and fluorescence excitations and separate the fluorescence emission and narrow bandpass filters limiting the excitation fluorescence spectrum.\n manufacturer: Doric Lenses\n\n	isosbestic_excitation_filter abc.BandOpticalFilter at 0x139967255132944\nFields:\n bandwidth_in_nm: 10.0\n center_wavelength_in_nm: 405.0\n description: Dual excitation band fiber photometry measurements use a Fluorescence Mini Cube with 4 ports: one port for the functional fluorescence excitation light, one for the isosbestic excitation, one for the fluorescence detection, and one for the sample. The cube has dichroic mirrors to combine isosbestic and fluorescence excitations and separate the fluorescence emission and narrow bandpass filters limiting the excitation fluorescence spectrum.\n filter_type: Bandpass\n manufacturer: Doric Lenses\n model: 4 ports Fluorescence Mini Cube - GCaMP pynwb.device.DeviceModel at 0x139967235670096\nFields:\n description: Dual excitation band fiber photometry measurements use a Fluorescence Mini Cube with 4 ports: one port for the functional fluorescence excitation light, one for the isosbestic excitation, one for the fluorescence detection, and one for the sample. The cube has dichroic mirrors to combine isosbestic and fluorescence excitations and separate the fluorescence emission and narrow bandpass filters limiting the excitation fluorescence spectrum.\n manufacturer: Doric Lenses\n\n

	location	indicator	optical_fiber	excitation_source	photodetector	dichroic_mirror	coordinates	commanded_voltage_series	emission_filter	excitation_filter
id
0	DMS	dms_green_fluorophore abc.Indicator at 0x139967255131024\nFields:\n description: Mice for fiber photometry experiments received infusions of 1ml of AAV5-CAG-FLEX-jGCaMP7b-WPRE (1.02e13 vg/mL, Addgene, lot 18-429) into lateral SNc (AP 3.1, ML 1.3, DV 4.2) in one hemisphere and medial SNc (AP 3.1, ML 0.8, DV 4.7) in the other. Hemispheres were counterbalanced between mice.\n injection_coordinates_in_mm: [3.1 0.8 4.7]\n injection_location: medial SNc\n label: GCaMP7b\n manufacturer: Addgene\n	optical_fiber abc.OpticalFiber at 0x139967234860976\nFields:\n core_diameter_in_um: 400.0\n description: Fiber optic implants (Doric Lenses; 400 um, 0.48 NA) were placed above DMS (AP 0.8, ML 1.5, DV 2.8) and DLS (AP 0.1, ML 2.8, DV 3.5). The DMS implant was placed in the hemisphere receiving a medial SNc viral injection, while the DLS implant was placed in the hemisphere receiving a lateral SNc viral injection. Calcium signals from dopamine terminals in DMS and DLS were recorded during RI30, on the first and last days of RI60/RR20 training as well as on both footshock probes for each mouse. All recordings were done using a fiber photometry rig with optical components from Doric lenses controlled by a real-time processor from Tucker Davis Technologies (TDT; RZ5P). TDT Synapse software was used for data acquisition.\n manufacturer: Doric Lenses\n model: Fiber Optic Implant pynwb.device.DeviceModel at 0x139967254695184\nFields:\n description: Fiber optic implants (Doric Lenses; 400 um, 0.48 NA) were placed above DMS (AP 0.8, ML 1.5, DV 2.8) and DLS (AP 0.1, ML 2.8, DV 3.5). The DMS implant was placed in the hemisphere receiving a medial SNc viral injection, while the DLS implant was placed in the hemisphere receiving a lateral SNc viral injection. Calcium signals from dopamine terminals in DMS and DLS were recorded during RI30, on the first and last days of RI60/RR20 training as well as on both footshock probes for each mouse. All recordings were done using a fiber photometry rig with optical components from Doric lenses controlled by a real-time processor from Tucker Davis Technologies (TDT; RZ5P). TDT Synapse software was used for data acquisition.\n manufacturer: Doric Lenses\n\n numerical_aperture: 0.48\n	excitation_source_calcium_signal abc.ExcitationSource at 0x139967234860640\nFields:\n description: 465nm and 405nm LEDs were modulated at 211 Hz and 330 Hz, respectively, for DMS probes. 465nm and 405nm LEDs were modulated at 450 Hz and 270 Hz, respectively for DLS probes. LED currents were adjusted in order to return a voltage between 150-200mV for each signal, were offset by 5 mA, were demodulated using a 4 Hz lowpass frequency filter.\n excitation_wavelength_in_nm: 465.0\n illumination_type: LED\n manufacturer: Doric Lenses\n model: Connectorized LED pynwb.device.DeviceModel at 0x139967254681312\nFields:\n description: 465nm and 405nm LEDs were modulated at 211 Hz and 330 Hz, respectively, for DMS probes. 465nm and 405nm LEDs were modulated at 450 Hz and 270 Hz, respectively for DLS probes. LED currents were adjusted in order to return a voltage between 150-200mV for each signal, were offset by 5 mA, were demodulated using a 4 Hz lowpass frequency filter.\n manufacturer: Doric Lenses\n\n	photodetector abc.Photodetector at 0x139967234861312\nFields:\n description: This battery-operated photoreceiver has high gain and detects CW light signals in the sub-picowatt to nanowatt range. When used in conjunction with a modulated light source and a lock-in amplifier to reduce the measurement bandwidth, it achieves sensitivity levels in the femtowatt range. Doric offer this Newport product with add-on fiber optic adapter that improves coupling efficiency between the large core, high NA optical fibers used in Fiber Photometry and relatively small detector area. Its output analog voltage (0-5 V) can be monitored with an oscilloscope or with a DAQ board to record the data with a computer.\n detected_wavelength_in_nm: 525.0\n detector_type: photodiode\n gain: 10000000000.0\n manufacturer: Doric Lenses\n model: Newport Visible Femtowatt Photoreceiver Module pynwb.device.DeviceModel at 0x139967254696272\nFields:\n description: This battery-operated photoreceiver has high gain and detects CW light signals in the sub-picowatt to nanowatt range. When used in conjunction with a modulated light source and a lock-in amplifier to reduce the measurement bandwidth, it achieves sensitivity levels in the femtowatt range. Doric offer this Newport product with add-on fiber optic adapter that improves coupling efficiency between the large core, high NA optical fibers used in Fiber Photometry and relatively small detector area. Its output analog voltage (0-5 V) can be monitored with an oscilloscope or with a DAQ board to record the data with a computer.\n manufacturer: Doric Lenses\n\n	dichroic_mirror abc.DichroicMirror at 0x139967234859632\nFields:\n description: Dual excitation band fiber photometry measurements use a Fluorescence Mini Cube with 4 ports: one port for the functional fluorescence excitation light, one for the isosbestic excitation, one for the fluorescence detection, and one for the sample. The cube has dichroic mirrors to combine isosbestic and fluorescence excitations and separate the fluorescence emission and narrow bandpass filters limiting the excitation fluorescence spectrum.\n manufacturer: Doric Lenses\n model: 4 ports Fluorescence Mini Cube - GCaMP pynwb.device.DeviceModel at 0x139967234859296\nFields:\n description: Dual excitation band fiber photometry measurements use a Fluorescence Mini Cube with 4 ports: one port for the functional fluorescence excitation light, one for the isosbestic excitation, one for the fluorescence detection, and one for the sample. The cube has dichroic mirrors to combine isosbestic and fluorescence excitations and separate the fluorescence emission and narrow bandpass filters limiting the excitation fluorescence spectrum.\n manufacturer: Doric Lenses\n\n	[0.8, 1.5, 2.8]	commanded_voltage_series_dms_calcium_signal abc.CommandedVoltageSeries at 0x139967255127184\nFields:\n comments: no comments\n conversion: 1.0\n data: <HDF5 dataset "data": shape (22570496,), type "<f4">\n description: The commanded voltage for the DMS calcium signal.\n frequency: 211.0\n frequency__unit: hertz\n offset: 0.0\n rate: 6103.515625\n resolution: -1.0\n starting_time: 0.0\n starting_time_unit: seconds\n unit: volts\n	emission_filter abc.BandOpticalFilter at 0x139967234860304\nFields:\n bandwidth_in_nm: 50.0\n center_wavelength_in_nm: 525.0\n description: Dual excitation band fiber photometry measurements use a Fluorescence Mini Cube with 4 ports: one port for the functional fluorescence excitation light, one for the isosbestic excitation, one for the fluorescence detection, and one for the sample. The cube has dichroic mirrors to combine isosbestic and fluorescence excitations and separate the fluorescence emission and narrow bandpass filters limiting the excitation fluorescence spectrum.\n filter_type: Bandpass\n manufacturer: Doric Lenses\n model: 4 ports Fluorescence Mini Cube - GCaMP pynwb.device.DeviceModel at 0x139967255131664\nFields:\n description: Dual excitation band fiber photometry measurements use a Fluorescence Mini Cube with 4 ports: one port for the functional fluorescence excitation light, one for the isosbestic excitation, one for the fluorescence detection, and one for the sample. The cube has dichroic mirrors to combine isosbestic and fluorescence excitations and separate the fluorescence emission and narrow bandpass filters limiting the excitation fluorescence spectrum.\n manufacturer: Doric Lenses\n\n	excitation_filter abc.BandOpticalFilter at 0x139967255132304\nFields:\n bandwidth_in_nm: 30.0\n center_wavelength_in_nm: 475.0\n description: Dual excitation band fiber photometry measurements use a Fluorescence Mini Cube with 4 ports: one port for the functional fluorescence excitation light, one for the isosbestic excitation, one for the fluorescence detection, and one for the sample. The cube has dichroic mirrors to combine isosbestic and fluorescence excitations and separate the fluorescence emission and narrow bandpass filters limiting the excitation fluorescence spectrum.\n filter_type: Bandpass\n manufacturer: Doric Lenses\n model: 4 ports Fluorescence Mini Cube - GCaMP pynwb.device.DeviceModel at 0x139967255131984\nFields:\n description: Dual excitation band fiber photometry measurements use a Fluorescence Mini Cube with 4 ports: one port for the functional fluorescence excitation light, one for the isosbestic excitation, one for the fluorescence detection, and one for the sample. The cube has dichroic mirrors to combine isosbestic and fluorescence excitations and separate the fluorescence emission and narrow bandpass filters limiting the excitation fluorescence spectrum.\n manufacturer: Doric Lenses\n\n
1	DMS	dms_green_fluorophore abc.Indicator at 0x139967255131024\nFields:\n description: Mice for fiber photometry experiments received infusions of 1ml of AAV5-CAG-FLEX-jGCaMP7b-WPRE (1.02e13 vg/mL, Addgene, lot 18-429) into lateral SNc (AP 3.1, ML 1.3, DV 4.2) in one hemisphere and medial SNc (AP 3.1, ML 0.8, DV 4.7) in the other. Hemispheres were counterbalanced between mice.\n injection_coordinates_in_mm: [3.1 0.8 4.7]\n injection_location: medial SNc\n label: GCaMP7b\n manufacturer: Addgene\n	optical_fiber abc.OpticalFiber at 0x139967234860976\nFields:\n core_diameter_in_um: 400.0\n description: Fiber optic implants (Doric Lenses; 400 um, 0.48 NA) were placed above DMS (AP 0.8, ML 1.5, DV 2.8) and DLS (AP 0.1, ML 2.8, DV 3.5). The DMS implant was placed in the hemisphere receiving a medial SNc viral injection, while the DLS implant was placed in the hemisphere receiving a lateral SNc viral injection. Calcium signals from dopamine terminals in DMS and DLS were recorded during RI30, on the first and last days of RI60/RR20 training as well as on both footshock probes for each mouse. All recordings were done using a fiber photometry rig with optical components from Doric lenses controlled by a real-time processor from Tucker Davis Technologies (TDT; RZ5P). TDT Synapse software was used for data acquisition.\n manufacturer: Doric Lenses\n model: Fiber Optic Implant pynwb.device.DeviceModel at 0x139967254695184\nFields:\n description: Fiber optic implants (Doric Lenses; 400 um, 0.48 NA) were placed above DMS (AP 0.8, ML 1.5, DV 2.8) and DLS (AP 0.1, ML 2.8, DV 3.5). The DMS implant was placed in the hemisphere receiving a medial SNc viral injection, while the DLS implant was placed in the hemisphere receiving a lateral SNc viral injection. Calcium signals from dopamine terminals in DMS and DLS were recorded during RI30, on the first and last days of RI60/RR20 training as well as on both footshock probes for each mouse. All recordings were done using a fiber photometry rig with optical components from Doric lenses controlled by a real-time processor from Tucker Davis Technologies (TDT; RZ5P). TDT Synapse software was used for data acquisition.\n manufacturer: Doric Lenses\n\n numerical_aperture: 0.48\n	excitation_source_isosbestic_control abc.ExcitationSource at 0x139967255132624\nFields:\n description: 465nm and 405nm LEDs were modulated at 211 Hz and 330 Hz, respectively, for DMS probes. 465nm and 405nm LEDs were modulated at 450 Hz and 270 Hz, respectively for DLS probes. LED currents were adjusted in order to return a voltage between 150-200mV for each signal, were offset by 5 mA, were demodulated using a 4 Hz lowpass frequency filter.\n excitation_wavelength_in_nm: 405.0\n illumination_type: LED\n manufacturer: Doric Lenses\n model: Connectorized LED pynwb.device.DeviceModel at 0x139967254681616\nFields:\n description: 465nm and 405nm LEDs were modulated at 211 Hz and 330 Hz, respectively, for DMS probes. 465nm and 405nm LEDs were modulated at 450 Hz and 270 Hz, respectively for DLS probes. LED currents were adjusted in order to return a voltage between 150-200mV for each signal, were offset by 5 mA, were demodulated using a 4 Hz lowpass frequency filter.\n manufacturer: Doric Lenses\n\n	photodetector abc.Photodetector at 0x139967234861312\nFields:\n description: This battery-operated photoreceiver has high gain and detects CW light signals in the sub-picowatt to nanowatt range. When used in conjunction with a modulated light source and a lock-in amplifier to reduce the measurement bandwidth, it achieves sensitivity levels in the femtowatt range. Doric offer this Newport product with add-on fiber optic adapter that improves coupling efficiency between the large core, high NA optical fibers used in Fiber Photometry and relatively small detector area. Its output analog voltage (0-5 V) can be monitored with an oscilloscope or with a DAQ board to record the data with a computer.\n detected_wavelength_in_nm: 525.0\n detector_type: photodiode\n gain: 10000000000.0\n manufacturer: Doric Lenses\n model: Newport Visible Femtowatt Photoreceiver Module pynwb.device.DeviceModel at 0x139967254696272\nFields:\n description: This battery-operated photoreceiver has high gain and detects CW light signals in the sub-picowatt to nanowatt range. When used in conjunction with a modulated light source and a lock-in amplifier to reduce the measurement bandwidth, it achieves sensitivity levels in the femtowatt range. Doric offer this Newport product with add-on fiber optic adapter that improves coupling efficiency between the large core, high NA optical fibers used in Fiber Photometry and relatively small detector area. Its output analog voltage (0-5 V) can be monitored with an oscilloscope or with a DAQ board to record the data with a computer.\n manufacturer: Doric Lenses\n\n	dichroic_mirror abc.DichroicMirror at 0x139967234859632\nFields:\n description: Dual excitation band fiber photometry measurements use a Fluorescence Mini Cube with 4 ports: one port for the functional fluorescence excitation light, one for the isosbestic excitation, one for the fluorescence detection, and one for the sample. The cube has dichroic mirrors to combine isosbestic and fluorescence excitations and separate the fluorescence emission and narrow bandpass filters limiting the excitation fluorescence spectrum.\n manufacturer: Doric Lenses\n model: 4 ports Fluorescence Mini Cube - GCaMP pynwb.device.DeviceModel at 0x139967234859296\nFields:\n description: Dual excitation band fiber photometry measurements use a Fluorescence Mini Cube with 4 ports: one port for the functional fluorescence excitation light, one for the isosbestic excitation, one for the fluorescence detection, and one for the sample. The cube has dichroic mirrors to combine isosbestic and fluorescence excitations and separate the fluorescence emission and narrow bandpass filters limiting the excitation fluorescence spectrum.\n manufacturer: Doric Lenses\n\n	[0.8, 1.5, 2.8]	commanded_voltage_series_dms_isosbestic_control abc.CommandedVoltageSeries at 0x139967254676448\nFields:\n comments: no comments\n conversion: 1.0\n data: <HDF5 dataset "data": shape (22570496,), type "<f4">\n description: The commanded voltage for the DMS isosbestic control.\n frequency: 330.0\n frequency__unit: hertz\n offset: 0.0\n rate: 6103.515625\n resolution: -1.0\n starting_time: 0.0\n starting_time_unit: seconds\n unit: volts\n	emission_filter abc.BandOpticalFilter at 0x139967234860304\nFields:\n bandwidth_in_nm: 50.0\n center_wavelength_in_nm: 525.0\n description: Dual excitation band fiber photometry measurements use a Fluorescence Mini Cube with 4 ports: one port for the functional fluorescence excitation light, one for the isosbestic excitation, one for the fluorescence detection, and one for the sample. The cube has dichroic mirrors to combine isosbestic and fluorescence excitations and separate the fluorescence emission and narrow bandpass filters limiting the excitation fluorescence spectrum.\n filter_type: Bandpass\n manufacturer: Doric Lenses\n model: 4 ports Fluorescence Mini Cube - GCaMP pynwb.device.DeviceModel at 0x139967255131664\nFields:\n description: Dual excitation band fiber photometry measurements use a Fluorescence Mini Cube with 4 ports: one port for the functional fluorescence excitation light, one for the isosbestic excitation, one for the fluorescence detection, and one for the sample. The cube has dichroic mirrors to combine isosbestic and fluorescence excitations and separate the fluorescence emission and narrow bandpass filters limiting the excitation fluorescence spectrum.\n manufacturer: Doric Lenses\n\n	isosbestic_excitation_filter abc.BandOpticalFilter at 0x139967255132944\nFields:\n bandwidth_in_nm: 10.0\n center_wavelength_in_nm: 405.0\n description: Dual excitation band fiber photometry measurements use a Fluorescence Mini Cube with 4 ports: one port for the functional fluorescence excitation light, one for the isosbestic excitation, one for the fluorescence detection, and one for the sample. The cube has dichroic mirrors to combine isosbestic and fluorescence excitations and separate the fluorescence emission and narrow bandpass filters limiting the excitation fluorescence spectrum.\n filter_type: Bandpass\n manufacturer: Doric Lenses\n model: 4 ports Fluorescence Mini Cube - GCaMP pynwb.device.DeviceModel at 0x139967235670096\nFields:\n description: Dual excitation band fiber photometry measurements use a Fluorescence Mini Cube with 4 ports: one port for the functional fluorescence excitation light, one for the isosbestic excitation, one for the fluorescence detection, and one for the sample. The cube has dichroic mirrors to combine isosbestic and fluorescence excitations and separate the fluorescence emission and narrow bandpass filters limiting the excitation fluorescence spectrum.\n manufacturer: Doric Lenses\n\n
2	DLS	dls_green_fluorophore abc.Indicator at 0x139967234859968\nFields:\n description: Mice for fiber photometry experiments received infusions of 1ml of AAV5-CAG-FLEX-jGCaMP7b-WPRE (1.02e13 vg/mL, Addgene, lot 18-429) into lateral SNc (AP 3.1, ML 1.3, DV 4.2) in one hemisphere and medial SNc (AP 3.1, ML 0.8, DV 4.7) in the other. Hemispheres were counterbalanced between mice.\n injection_coordinates_in_mm: [3.1 1.3 4.2]\n injection_location: lateral SNc\n label: GCaMP7b\n manufacturer: Addgene\n	optical_fiber abc.OpticalFiber at 0x139967234860976\nFields:\n core_diameter_in_um: 400.0\n description: Fiber optic implants (Doric Lenses; 400 um, 0.48 NA) were placed above DMS (AP 0.8, ML 1.5, DV 2.8) and DLS (AP 0.1, ML 2.8, DV 3.5). The DMS implant was placed in the hemisphere receiving a medial SNc viral injection, while the DLS implant was placed in the hemisphere receiving a lateral SNc viral injection. Calcium signals from dopamine terminals in DMS and DLS were recorded during RI30, on the first and last days of RI60/RR20 training as well as on both footshock probes for each mouse. All recordings were done using a fiber photometry rig with optical components from Doric lenses controlled by a real-time processor from Tucker Davis Technologies (TDT; RZ5P). TDT Synapse software was used for data acquisition.\n manufacturer: Doric Lenses\n model: Fiber Optic Implant pynwb.device.DeviceModel at 0x139967254695184\nFields:\n description: Fiber optic implants (Doric Lenses; 400 um, 0.48 NA) were placed above DMS (AP 0.8, ML 1.5, DV 2.8) and DLS (AP 0.1, ML 2.8, DV 3.5). The DMS implant was placed in the hemisphere receiving a medial SNc viral injection, while the DLS implant was placed in the hemisphere receiving a lateral SNc viral injection. Calcium signals from dopamine terminals in DMS and DLS were recorded during RI30, on the first and last days of RI60/RR20 training as well as on both footshock probes for each mouse. All recordings were done using a fiber photometry rig with optical components from Doric lenses controlled by a real-time processor from Tucker Davis Technologies (TDT; RZ5P). TDT Synapse software was used for data acquisition.\n manufacturer: Doric Lenses\n\n numerical_aperture: 0.48\n	excitation_source_calcium_signal abc.ExcitationSource at 0x139967234860640\nFields:\n description: 465nm and 405nm LEDs were modulated at 211 Hz and 330 Hz, respectively, for DMS probes. 465nm and 405nm LEDs were modulated at 450 Hz and 270 Hz, respectively for DLS probes. LED currents were adjusted in order to return a voltage between 150-200mV for each signal, were offset by 5 mA, were demodulated using a 4 Hz lowpass frequency filter.\n excitation_wavelength_in_nm: 465.0\n illumination_type: LED\n manufacturer: Doric Lenses\n model: Connectorized LED pynwb.device.DeviceModel at 0x139967254681312\nFields:\n description: 465nm and 405nm LEDs were modulated at 211 Hz and 330 Hz, respectively, for DMS probes. 465nm and 405nm LEDs were modulated at 450 Hz and 270 Hz, respectively for DLS probes. LED currents were adjusted in order to return a voltage between 150-200mV for each signal, were offset by 5 mA, were demodulated using a 4 Hz lowpass frequency filter.\n manufacturer: Doric Lenses\n\n	photodetector abc.Photodetector at 0x139967234861312\nFields:\n description: This battery-operated photoreceiver has high gain and detects CW light signals in the sub-picowatt to nanowatt range. When used in conjunction with a modulated light source and a lock-in amplifier to reduce the measurement bandwidth, it achieves sensitivity levels in the femtowatt range. Doric offer this Newport product with add-on fiber optic adapter that improves coupling efficiency between the large core, high NA optical fibers used in Fiber Photometry and relatively small detector area. Its output analog voltage (0-5 V) can be monitored with an oscilloscope or with a DAQ board to record the data with a computer.\n detected_wavelength_in_nm: 525.0\n detector_type: photodiode\n gain: 10000000000.0\n manufacturer: Doric Lenses\n model: Newport Visible Femtowatt Photoreceiver Module pynwb.device.DeviceModel at 0x139967254696272\nFields:\n description: This battery-operated photoreceiver has high gain and detects CW light signals in the sub-picowatt to nanowatt range. When used in conjunction with a modulated light source and a lock-in amplifier to reduce the measurement bandwidth, it achieves sensitivity levels in the femtowatt range. Doric offer this Newport product with add-on fiber optic adapter that improves coupling efficiency between the large core, high NA optical fibers used in Fiber Photometry and relatively small detector area. Its output analog voltage (0-5 V) can be monitored with an oscilloscope or with a DAQ board to record the data with a computer.\n manufacturer: Doric Lenses\n\n	dichroic_mirror abc.DichroicMirror at 0x139967234859632\nFields:\n description: Dual excitation band fiber photometry measurements use a Fluorescence Mini Cube with 4 ports: one port for the functional fluorescence excitation light, one for the isosbestic excitation, one for the fluorescence detection, and one for the sample. The cube has dichroic mirrors to combine isosbestic and fluorescence excitations and separate the fluorescence emission and narrow bandpass filters limiting the excitation fluorescence spectrum.\n manufacturer: Doric Lenses\n model: 4 ports Fluorescence Mini Cube - GCaMP pynwb.device.DeviceModel at 0x139967234859296\nFields:\n description: Dual excitation band fiber photometry measurements use a Fluorescence Mini Cube with 4 ports: one port for the functional fluorescence excitation light, one for the isosbestic excitation, one for the fluorescence detection, and one for the sample. The cube has dichroic mirrors to combine isosbestic and fluorescence excitations and separate the fluorescence emission and narrow bandpass filters limiting the excitation fluorescence spectrum.\n manufacturer: Doric Lenses\n\n	[0.1, 2.8, 3.5]	commanded_voltage_series_dls_calcium_signal abc.CommandedVoltageSeries at 0x139967234853584\nFields:\n comments: no comments\n conversion: 1.0\n data: <HDF5 dataset "data": shape (22570496,), type "<f4">\n description: The commanded voltage for the DLS calcium signal.\n frequency: 450.0\n frequency__unit: hertz\n offset: 0.0\n rate: 6103.515625\n resolution: -1.0\n starting_time: 0.0\n starting_time_unit: seconds\n unit: volts\n	emission_filter abc.BandOpticalFilter at 0x139967234860304\nFields:\n bandwidth_in_nm: 50.0\n center_wavelength_in_nm: 525.0\n description: Dual excitation band fiber photometry measurements use a Fluorescence Mini Cube with 4 ports: one port for the functional fluorescence excitation light, one for the isosbestic excitation, one for the fluorescence detection, and one for the sample. The cube has dichroic mirrors to combine isosbestic and fluorescence excitations and separate the fluorescence emission and narrow bandpass filters limiting the excitation fluorescence spectrum.\n filter_type: Bandpass\n manufacturer: Doric Lenses\n model: 4 ports Fluorescence Mini Cube - GCaMP pynwb.device.DeviceModel at 0x139967255131664\nFields:\n description: Dual excitation band fiber photometry measurements use a Fluorescence Mini Cube with 4 ports: one port for the functional fluorescence excitation light, one for the isosbestic excitation, one for the fluorescence detection, and one for the sample. The cube has dichroic mirrors to combine isosbestic and fluorescence excitations and separate the fluorescence emission and narrow bandpass filters limiting the excitation fluorescence spectrum.\n manufacturer: Doric Lenses\n\n	excitation_filter abc.BandOpticalFilter at 0x139967255132304\nFields:\n bandwidth_in_nm: 30.0\n center_wavelength_in_nm: 475.0\n description: Dual excitation band fiber photometry measurements use a Fluorescence Mini Cube with 4 ports: one port for the functional fluorescence excitation light, one for the isosbestic excitation, one for the fluorescence detection, and one for the sample. The cube has dichroic mirrors to combine isosbestic and fluorescence excitations and separate the fluorescence emission and narrow bandpass filters limiting the excitation fluorescence spectrum.\n filter_type: Bandpass\n manufacturer: Doric Lenses\n model: 4 ports Fluorescence Mini Cube - GCaMP pynwb.device.DeviceModel at 0x139967255131984\nFields:\n description: Dual excitation band fiber photometry measurements use a Fluorescence Mini Cube with 4 ports: one port for the functional fluorescence excitation light, one for the isosbestic excitation, one for the fluorescence detection, and one for the sample. The cube has dichroic mirrors to combine isosbestic and fluorescence excitations and separate the fluorescence emission and narrow bandpass filters limiting the excitation fluorescence spectrum.\n manufacturer: Doric Lenses\n\n
3	DLS	dls_green_fluorophore abc.Indicator at 0x139967234859968\nFields:\n description: Mice for fiber photometry experiments received infusions of 1ml of AAV5-CAG-FLEX-jGCaMP7b-WPRE (1.02e13 vg/mL, Addgene, lot 18-429) into lateral SNc (AP 3.1, ML 1.3, DV 4.2) in one hemisphere and medial SNc (AP 3.1, ML 0.8, DV 4.7) in the other. Hemispheres were counterbalanced between mice.\n injection_coordinates_in_mm: [3.1 1.3 4.2]\n injection_location: lateral SNc\n label: GCaMP7b\n manufacturer: Addgene\n	optical_fiber abc.OpticalFiber at 0x139967234860976\nFields:\n core_diameter_in_um: 400.0\n description: Fiber optic implants (Doric Lenses; 400 um, 0.48 NA) were placed above DMS (AP 0.8, ML 1.5, DV 2.8) and DLS (AP 0.1, ML 2.8, DV 3.5). The DMS implant was placed in the hemisphere receiving a medial SNc viral injection, while the DLS implant was placed in the hemisphere receiving a lateral SNc viral injection. Calcium signals from dopamine terminals in DMS and DLS were recorded during RI30, on the first and last days of RI60/RR20 training as well as on both footshock probes for each mouse. All recordings were done using a fiber photometry rig with optical components from Doric lenses controlled by a real-time processor from Tucker Davis Technologies (TDT; RZ5P). TDT Synapse software was used for data acquisition.\n manufacturer: Doric Lenses\n model: Fiber Optic Implant pynwb.device.DeviceModel at 0x139967254695184\nFields:\n description: Fiber optic implants (Doric Lenses; 400 um, 0.48 NA) were placed above DMS (AP 0.8, ML 1.5, DV 2.8) and DLS (AP 0.1, ML 2.8, DV 3.5). The DMS implant was placed in the hemisphere receiving a medial SNc viral injection, while the DLS implant was placed in the hemisphere receiving a lateral SNc viral injection. Calcium signals from dopamine terminals in DMS and DLS were recorded during RI30, on the first and last days of RI60/RR20 training as well as on both footshock probes for each mouse. All recordings were done using a fiber photometry rig with optical components from Doric lenses controlled by a real-time processor from Tucker Davis Technologies (TDT; RZ5P). TDT Synapse software was used for data acquisition.\n manufacturer: Doric Lenses\n\n numerical_aperture: 0.48\n	excitation_source_isosbestic_control abc.ExcitationSource at 0x139967255132624\nFields:\n description: 465nm and 405nm LEDs were modulated at 211 Hz and 330 Hz, respectively, for DMS probes. 465nm and 405nm LEDs were modulated at 450 Hz and 270 Hz, respectively for DLS probes. LED currents were adjusted in order to return a voltage between 150-200mV for each signal, were offset by 5 mA, were demodulated using a 4 Hz lowpass frequency filter.\n excitation_wavelength_in_nm: 405.0\n illumination_type: LED\n manufacturer: Doric Lenses\n model: Connectorized LED pynwb.device.DeviceModel at 0x139967254681616\nFields:\n description: 465nm and 405nm LEDs were modulated at 211 Hz and 330 Hz, respectively, for DMS probes. 465nm and 405nm LEDs were modulated at 450 Hz and 270 Hz, respectively for DLS probes. LED currents were adjusted in order to return a voltage between 150-200mV for each signal, were offset by 5 mA, were demodulated using a 4 Hz lowpass frequency filter.\n manufacturer: Doric Lenses\n\n	photodetector abc.Photodetector at 0x139967234861312\nFields:\n description: This battery-operated photoreceiver has high gain and detects CW light signals in the sub-picowatt to nanowatt range. When used in conjunction with a modulated light source and a lock-in amplifier to reduce the measurement bandwidth, it achieves sensitivity levels in the femtowatt range. Doric offer this Newport product with add-on fiber optic adapter that improves coupling efficiency between the large core, high NA optical fibers used in Fiber Photometry and relatively small detector area. Its output analog voltage (0-5 V) can be monitored with an oscilloscope or with a DAQ board to record the data with a computer.\n detected_wavelength_in_nm: 525.0\n detector_type: photodiode\n gain: 10000000000.0\n manufacturer: Doric Lenses\n model: Newport Visible Femtowatt Photoreceiver Module pynwb.device.DeviceModel at 0x139967254696272\nFields:\n description: This battery-operated photoreceiver has high gain and detects CW light signals in the sub-picowatt to nanowatt range. When used in conjunction with a modulated light source and a lock-in amplifier to reduce the measurement bandwidth, it achieves sensitivity levels in the femtowatt range. Doric offer this Newport product with add-on fiber optic adapter that improves coupling efficiency between the large core, high NA optical fibers used in Fiber Photometry and relatively small detector area. Its output analog voltage (0-5 V) can be monitored with an oscilloscope or with a DAQ board to record the data with a computer.\n manufacturer: Doric Lenses\n\n	dichroic_mirror abc.DichroicMirror at 0x139967234859632\nFields:\n description: Dual excitation band fiber photometry measurements use a Fluorescence Mini Cube with 4 ports: one port for the functional fluorescence excitation light, one for the isosbestic excitation, one for the fluorescence detection, and one for the sample. The cube has dichroic mirrors to combine isosbestic and fluorescence excitations and separate the fluorescence emission and narrow bandpass filters limiting the excitation fluorescence spectrum.\n manufacturer: Doric Lenses\n model: 4 ports Fluorescence Mini Cube - GCaMP pynwb.device.DeviceModel at 0x139967234859296\nFields:\n description: Dual excitation band fiber photometry measurements use a Fluorescence Mini Cube with 4 ports: one port for the functional fluorescence excitation light, one for the isosbestic excitation, one for the fluorescence detection, and one for the sample. The cube has dichroic mirrors to combine isosbestic and fluorescence excitations and separate the fluorescence emission and narrow bandpass filters limiting the excitation fluorescence spectrum.\n manufacturer: Doric Lenses\n\n	[0.1, 2.8, 3.5]	commanded_voltage_series_dls_isosbestic_control abc.CommandedVoltageSeries at 0x139967255126544\nFields:\n comments: no comments\n conversion: 1.0\n data: <HDF5 dataset "data": shape (22570496,), type "<f4">\n description: The commanded voltage for the DLS isosbestic control.\n frequency: 270.0\n frequency__unit: hertz\n offset: 0.0\n rate: 6103.515625\n resolution: -1.0\n starting_time: 0.0\n starting_time_unit: seconds\n unit: volts\n	emission_filter abc.BandOpticalFilter at 0x139967234860304\nFields:\n bandwidth_in_nm: 50.0\n center_wavelength_in_nm: 525.0\n description: Dual excitation band fiber photometry measurements use a Fluorescence Mini Cube with 4 ports: one port for the functional fluorescence excitation light, one for the isosbestic excitation, one for the fluorescence detection, and one for the sample. The cube has dichroic mirrors to combine isosbestic and fluorescence excitations and separate the fluorescence emission and narrow bandpass filters limiting the excitation fluorescence spectrum.\n filter_type: Bandpass\n manufacturer: Doric Lenses\n model: 4 ports Fluorescence Mini Cube - GCaMP pynwb.device.DeviceModel at 0x139967255131664\nFields:\n description: Dual excitation band fiber photometry measurements use a Fluorescence Mini Cube with 4 ports: one port for the functional fluorescence excitation light, one for the isosbestic excitation, one for the fluorescence detection, and one for the sample. The cube has dichroic mirrors to combine isosbestic and fluorescence excitations and separate the fluorescence emission and narrow bandpass filters limiting the excitation fluorescence spectrum.\n manufacturer: Doric Lenses\n\n	isosbestic_excitation_filter abc.BandOpticalFilter at 0x139967255132944\nFields:\n bandwidth_in_nm: 10.0\n center_wavelength_in_nm: 405.0\n description: Dual excitation band fiber photometry measurements use a Fluorescence Mini Cube with 4 ports: one port for the functional fluorescence excitation light, one for the isosbestic excitation, one for the fluorescence detection, and one for the sample. The cube has dichroic mirrors to combine isosbestic and fluorescence excitations and separate the fluorescence emission and narrow bandpass filters limiting the excitation fluorescence spectrum.\n filter_type: Bandpass\n manufacturer: Doric Lenses\n model: 4 ports Fluorescence Mini Cube - GCaMP pynwb.device.DeviceModel at 0x139967235670096\nFields:\n description: Dual excitation band fiber photometry measurements use a Fluorescence Mini Cube with 4 ports: one port for the functional fluorescence excitation light, one for the isosbestic excitation, one for the fluorescence detection, and one for the sample. The cube has dichroic mirrors to combine isosbestic and fluorescence excitations and separate the fluorescence emission and narrow bandpass filters limiting the excitation fluorescence spectrum.\n manufacturer: Doric Lenses\n\n